ArticleAdvanced pharmaceutical bulletin2013
The effects of lyophilization on the physico-chemical stability of sirolimus liposomes.
Article in Advanced pharmaceutical bulletin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
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- Stepwise effects of formulation additives and process parameters on ethanol injection-prepared nanoliposomes for cancer delivery: a preformulation study.Scientific reports · 2026Article
- Silibinin-Loaded Liposomes: The Influence of Modifications on Physicochemical Characteristics, Stability, and Bioactivity Associated with Dermal Application.Pharmaceutics · 2024Article
- Mesenchymal stem cell membrane-coated nanoconstructs: why have they not yet found a home in clinical practice?Nanomedicine (London, England) · 2024Article
- Freeze-drying of bupivacaine lipospheres: preparation, characterization, and evaluation of anti-microbial properties.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2024Article
- Atorvastatin liposomes in a 3D-printed polymer film: a repurposing approach for local treatment of oral candidiasis.Drug delivery and translational research · 2023Article
- Liposomes for Tumor Targeted Therapy: A Review.International journal of molecular sciences · 2023Review
- Preparation, Characterization, Wound Healing, and Cytotoxicity Assay of PEGylated Nanophytosomes Loaded with 6-Gingerol.Nutrients · 2022Article
- A facile method for anti-cancer drug encapsulation into polymersomes with a core-satellite structure.Drug delivery · 2022Article
- Hybrid Self-Assembling Nanoparticles Encapsulating Zoledronic Acid: A Strategy for Fostering Their Clinical Use.International journal of molecular sciences · 2022Article
- Thermotropic effects of PEGylated lipids on the stability of HPPH-encapsulated lipid nanoparticles (LNP).Journal of thermal analysis and calorimetry · 2022Article
- Liposomes: Structure, Biomedical Applications, and Stability Parameters With Emphasis on Cholesterol.Frontiers in bioengineering and biotechnology · 2021Review
- Sugar and Polymer Excipients Enhance Uptake and Splice-Switching Activity of Peptide-Dendrimer/Lipid/Oligonucleotide Formulations.Pharmaceutics · 2019Article
- Selected Physicochemical Properties of Lyophilized Hydrogel with Liposomal Fraction of Calcium Dobesilate.Materials (Basel, Switzerland) · 2018Article
- Liposomal Drug Delivery Systems and Anticancer Drugs.Molecules (Basel, Switzerland) · 2018Review
- SATB1 siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells.OncoTargets and therapy · 2018Article
- Aggregated Nanotransfersomal Dry Powder Inhalation of Itraconazole for Pulmonary Drug Delivery.Advanced pharmaceutical bulletin · 2016Article
- Article
- One Step Encapsulation of Small Molecule Drugs in Liposomes via Electrospray-Remote Loading.Molecular pharmaceutics · 2016Article
- Nanoethosomes for Dermal Delivery of Lidocaine.Advanced pharmaceutical bulletin · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe major limitation in the widespread use of liposome drug delivery system is its instability. Lyophilization is a promising approach to ensure the long-term stability of liposomes. The aim of this study was to prepare sirolimus-loaded liposomes, study their stability and investigate the effect of lyophilization either in the presence or in the absence of lyoprotectant on liposome properties.
methodsTwo types of multi-lamellar liposomes, conventional and fusogenic, containing sirolimus were prepared by modified thin film hydration method with different ratio of dipalmitoylphosphatidylcholine (DPPC), cholesterol and dioleoylphosphoethanolamine (DOPE), and were lyophilized with or without dextrose as lyoprotectant. Chemical stability investigation was performed at 4°C and 25°C until 6 months using a validated HPLC method. Physical stability was studied with determination of particle size (PS) and encapsulation efficiency (EE %) of formulations through 6 months.
resultsChemical stability test at 4°C and 25°C until 6 months showed that drug content of liposomes decreased 8.4% and 20.2% respectively. Initial mean EE % and PS were 72.8 % and 582 nm respectively. After 6 months mean EE % for suspended form, lyophilized without lyoprotectant and lyophilized with lyoprotectant were 54.8 %, 62.3% and 67.1 % at 4°C and 48.2%, 60.4 % and 66.8 % at 25°C respectively. Corresponding data for mean PS were 8229 nm, 2397 nm and 688nm at 4°C and 9362 nm, 1944 nm and 737 nm at 25°C respectively.
conclusionIt is concluded that lyophilization with and without dextrose could increase shelf life of liposome and dextrose has lyoprotectant effect that stabilized liposomes in the lyophilization process.
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