ArticlePloS one2013
Transcriptome analysis of Portunus trituberculatus in response to salinity stress provides insights into the molecular basis of osmoregulation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 140 citations in OpenAlex.
- Here We Are, But Where Do We Go? A Systematic Review of Crustacean Transcriptomic Studies from 2014-2015.Integrative and comparative biology · 2016Pooled it
- Assessing Molecular Mechanisms of Stress Induced Salinity Adaptation in the Juvenile Ornate Spiny Lobster,International journal of molecular sciences · 2025Article
- De Novo Transcriptome Assembly Reveals Insights Into Osmoregulation and Oxidative Stress Response in the Gills of the Southern King Crab (Ecology and evolution · 2025Article
- An Integrated Transcriptomic and Proteomic Approach Uncovers the Molecular Mechanisms of Hypoosmotic Adaptation inInternational journal of molecular sciences · 2025Article
- Effects of the plant growth-promoting rhizobacterium Zobellella sp. DQSA1 on alleviating salt-alkali stress in job's tears seedings and its growth-promoting mechanism.BMC plant biology · 2025Article
- Analysis of adaptive molecular mechanisms in response to low salinity in antennal gland of mud crab,Heliyon · 2024Article
- Comparative Transcriptome Analysis of the Response toBiology · 2023Article
- A Multi-Species Comparison and Evolutionary Perspectives on Ion Regulation in the Antennal Gland of Brachyurans.Frontiers in physiology · 2022Review
- Evaluation of Krill Meal in Commercial Diets for Juvenile Swimming Crab (Aquaculture nutrition · 2022Article
- Genome-Wide Identification, Characterization, and Expression Analysis of DDE_Tnp_4 Family Genes inAntibiotics (Basel, Switzerland) · 2021Article
- Invasion of the body snatchers: the role of parasite introduction in host distribution and response to salinity in invaded estuaries.Proceedings. Biological sciences · 2021Article
- Characterization ofFrontiers in physiology · 2021Article
- Multi-omic approach provides insights into osmoregulation and osmoconformation of the crab Scylla paramamosain.Scientific reports · 2020Article
- Hemolymph composition, gene expressions in the gills, and thus the survival of euryhaline crabs are controlled by ambient minor cations according to osmotic condition-dependent manner.Ecology and evolution · 2020Article
- Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male).BMC genomics · 2020Article
- Chromosome-level genome assembly reveals the unique genome evolution of the swimming crab (Portunus trituberculatus).GigaScience · 2020Article
- Disparate responses to salinity across species and organizational levels in anchialine shrimps.The Journal of experimental biology · 2019Article
- The Single-molecule long-read sequencing of Scylla paramamosain.Scientific reports · 2019Article
- Transcriptomic analysis provides insight into the mechanism of salinity adjustment in swimming crab Portunus trituberculatus.Genes & genomics · 2019Article
- Discovery of Novel Crustacean and Cephalopod Flaviviruses: Insights into the Evolution and Circulation of Flaviviruses between Marine Invertebrate and Vertebrate Hosts.Journal of virology · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe swimming crab, Portunus trituberculatus, which is naturally distributed in the coastal waters of Asia-Pacific countries, is an important farmed species in China. Salinity is one of the most important abiotic factors that influence not only the distribution and abundance of crustaceans, it is also an important factor for artificial propagation of the crab. To better understand the interaction between salinity stress and osmoregulation, we performed a transcriptome analysis in the gills of Portunus trituberculatus challenged with salinity stress, using the Illumina Deep Sequencing technology.
resultsWe obtained 27,696,835, 28,268,353 and 33,901,271 qualified Illumina read pairs from low salinity challenged (LC), non-challenged (NC), and high salinity challenged (HC) Portunus trituberculatus cDNA libraries, respectively. The overall de novo assembly of cDNA sequence data generated 94,511 unigenes, with an average length of 644 bp. Comparative genomic analysis revealed that 1,705 genes differentially expressed in salinity stress compared to the controls, including 615 and 1,516 unigenes in NC vs LC and NC vs HC respectively. GO functional enrichment analysis results showed some differentially expressed genes were involved in crucial processes related to osmoregulation, such as ion transport processes, amino acid metabolism and synthesis processes, proteolysis process and chitin metabolic process.
conclusionThis work represents the first report of the utilization of the next generation sequencing techniques for transcriptome analysis in Portunus trituberculatus and provides valuable information on salinity adaptation mechanism. Results reveal a substantial number of genes modified by salinity stress and a few important salinity acclimation pathways, which will serve as an invaluable resource for revealing the molecular basis of osmoregulation in Portunus trituberculatus. In addition, the most comprehensive sequences of transcripts reported in this study provide a rich source for identification of novel genes in the crab.
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