ArticlePloS one2013
MagFRET: the first genetically encoded fluorescent Mg2+ sensor.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Structural and mutational insights define ERMA as the ER MgScience advances · 2026Article
- Ion channel and biophysical properties of extracellular vesicles.The Journal of biological chemistry · 2026Review
- CST1 Regulates ER-Mitochondrial Mg²⁺ Balance via MAGT1 to Promote Breast Cancer Metabolism and Growth.International journal of biological sciences · 2026Article
- TUSC3 regulates ERMA-mediated MgNature communications · 2025Article
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- CMOS-Based Implantable Multi-Ion Image Sensor for MgSensors (Basel, Switzerland) · 2025Article
- MgScience advances · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- ERMA (TMEM94) is a P-type ATPase transporter for MgMolecular cell · 2024Article
- Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.Plant physiology · 2023Review
- Genetically encoded fluorescent sensors for metals in biology.Current opinion in chemical biology · 2023Review
- PRL-1/2 phosphatases control TRPM7 magnesium-dependent function to regulate cellular bioenergetics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation.Cell reports methods · 2023Article
- A genetically encoded fluorescent sensor for manganese(II), engineered from lanmodulin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fluorescent Indicators For Biological Imaging of Monatomic Ions.Frontiers in cell and developmental biology · 2022Review
- Article
- The design and evolution of fluorescent protein-based sensors for monoatomic ions in biology.Protein engineering, design & selection : PEDS · 2021Review
- Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory.eLife · 2020Article
- Advances in imaging of understudied ions in signaling: A focus on magnesium.Current opinion in chemical biology · 2020Review
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5 authors.
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Abstract
Magnesium has important structural, catalytic and signaling roles in cells, yet few tools exist to image this metal ion in real time and at subcellular resolution. Here we report the first genetically encoded sensor for Mg(2+), MagFRET-1. This sensor is based on the high-affinity Mg(2+) binding domain of human centrin 3 (HsCen3), which undergoes a transition from a molten-globular apo form to a compactly-folded Mg(2+)-bound state. Fusion of Cerulean and Citrine fluorescent domains to the ends of HsCen3, yielded MagFRET-1, which combines a physiologically relevant Mg(2+) affinity (K d = 148 µM) with a 50% increase in emission ratio upon Mg(2+) binding due to a change in FRET efficiency between Cerulean and Citrine. Mutations in the metal binding sites yielded MagFRET variants whose Mg(2+) affinities were attenuated 2- to 100-fold relative to MagFRET-1, thus covering a broad range of Mg(2+) concentrations. In situ experiments in HEK293 cells showed that MagFRET-1 can be targeted to the cytosol and the nucleus. Clear responses to changes in extracellular Mg(2+) concentration were observed for MagFRET-1-expressing HEK293 cells when they were permeabilized with digitonin, whereas similar changes were not observed for intact cells. Although MagFRET-1 is also sensitive to Ca(2+), this affinity is sufficiently attenuated (K d of 10 µM) to make the sensor insensitive to known Ca(2+) stimuli in HEK293 cells. While the potential and limitations of the MagFRET sensors for intracellular Mg(2+) imaging need to be further established, we expect that these genetically encoded and ratiometric fluorescent Mg(2+) sensors could prove very useful in understanding intracellular Mg(2+) homeostasis and signaling.
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