ArticlePloS one2013
Next generation sequencing uncovers unexpected bacterial pathogens in ticks in western Europe.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Trends and Opportunities in Tick-Borne Disease Geography.Journal of medical entomology · 2021Pooled it
- Tick Microbiome and Its Role in Emerging Zoonotic Diseases and Transmissibility.Microorganisms · 2026Review
- Analysis of tick-borne pathogens using next-generation sequencing in ticks from wild animals in the Republic of Korea.Journal of veterinary science · 2025Article
- No evidence of Bartonella infections in host-seeking Ixodes scapularis and Ixodes pacificus ticks in the United States.Parasites & vectors · 2024Article
- Challenges for ticks and tick-borne diseases research in Southeast Asia: Insight from the first international symposium in Cambodia.PLoS neglected tropical diseases · 2024Review
- Prevalence of Lyme Disease and Relapsing FeverPathogens (Basel, Switzerland) · 2024Review
- Microbes and Mental Illness: Past, Present, and Future.Healthcare (Basel, Switzerland) · 2023Review
- Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers.Parasites & vectors · 2023Article
- Characterization of the bacterial microbiome of Swedish ticks through 16S rRNA amplicon sequencing of whole ticks and of individual tick organs.Parasites & vectors · 2023Article
- A Search for Tick-Associated, Bronnoya-like Virus Spillover into Sheep.Microorganisms · 2023Article
- mNGS helped diagnose scrub typhus presenting as a urinary tract infection with high D-dimer levels: a case report.BMC infectious diseases · 2021Article
- The Isolation of Culturable Bacteria inInternational journal of environmental research and public health · 2021Article
- Investigation of Tick-Borne Pathogens inPathogens (Basel, Switzerland) · 2021Article
- Multi-country investigation of the diversity and associated microorganisms isolated from tick species from domestic animals, wildlife and vegetation in selected african countries.Experimental & applied acarology · 2021Article
- High Diversity, Prevalence, and Co-infection Rates of Tick-Borne Pathogens in Ticks and Wildlife Hosts in an Urban Area in Romania.Frontiers in microbiology · 2021Article
- Induced Transient Immune Tolerance in Ticks and Vertebrate Host: A Keystone of Tick-Borne Diseases?Frontiers in immunology · 2021Review
- Interactive Gene Expression BetweenFrontiers in physiology · 2021Article
- Evaluation of a novel multiplex PCR amplicon sequencing assay for detection of human pathogens in Ixodes ticks.Ticks and tick-borne diseases · 2020Article
- Enhanced threat of tick-borne infections within cities? Assessing public health risks due to ticks in urban green spaces in Helsinki, Finland.Zoonoses and public health · 2020Article
- Failed Disruption of Tick Feeding, Viability, and Molting after Immunization of Mice and Sheep with RecombinantVaccines · 2020Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsTicks are highly susceptible to global environmental and socio-economical changes. Several tick-borne pathogens have been reported in new geographical regions while new species, strains or genetic variants of tick-borne microorganisms are continually being detected. However, tick-borne pathogens are still poorly understood, and it is estimated that half of all human tick-borne disease has an unknown origin. Therefore in order to prevent these diseases, more effort is required to identify unknown or unexpected tick-borne pathogens. Ixodes ricinus is the vector for a broad range of bacterial pathogens and the most prevalent tick in Europe. The aim of the present study was to evaluate the capability of Next Generation Sequencing (NGS) to extend the inventory of pathogenic bacteria carried by this species of tick in France.
methodsRNA and DNA were extracted from 1450 I. ricinus questing nymphs collected by flagging in Alsace, France. RNA was pooled and used for NGS. Following de novo assembly, bacterial contigs were assigned to the closest known taxonomy. DNA was used for real time PCR to confirm taxonomic species assignment of NGS-derived contigs for the doubtful cases, and for determination of prevalence.
resultsWe have generated a global in-depth picture of tick-borne bacteria. We identified RNA from the main pathogenic bacterial species known to be transmitted by I. ricinus. In addition we also identified unanticipated bacterial species for which we have estimated the prevalence within those ticks inhabiting the studied areas.
conclusionsThe data obtained from this study has proven that NGS has an enormous potential to detect the unexpected and provides the means to monitor pathogen occurrence.
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