ArticlePloS one2013
Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Review
- Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight.Scientific reports · 2022Article
- Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size ofFrontiers in cell and developmental biology · 2022Article
- Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Virus matrix interference on assessment of virucidal activity of high-touch surfaces designed to prevent hospital-acquired infections.Antimicrobial resistance and infection control · 2021Article
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires.Biophysical journal · 2021Article
- Biophysical Parameters of GEVIs: Considerations for Imaging Voltage.Biophysical journal · 2020Review
- Integrative analysis suggests cell type-specific decoding of NF-κB dynamics.Science signaling · 2020Article
- Review
- Genetic voltage indicators.BMC biology · 2019Review
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Insights from intoxicated Drosophila.Alcohol (Fayetteville, N.Y.) · 2019Review
- Optimizing Strategies for Developing Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice.Scientific reports · 2018Article
- Article
- Voltage and Calcium Imaging of Brain Activity.Biophysical journal · 2017Review
- Modulating the Voltage-sensitivity of a Genetically Encoded Voltage Indicator.Experimental neurobiology · 2017Article
- Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We previously reported the discovery of a fluorescent protein voltage probe, ArcLight, and its derivatives that exhibit large changes in fluorescence intensity in response to changes of plasma membrane voltage. ArcLight allows the reliable detection of single action potentials and sub-threshold activities in individual neurons and dendrites. The response kinetics of ArcLight (τ1-on ~10 ms, τ2-on ~ 50 ms) are comparable with most published genetically-encoded voltage probes. However, probes using voltage-sensing domains other than that from the Ciona intestinalis voltage sensitive phosphatase exhibit faster kinetics. Here we report new versions of ArcLight, in which the Ciona voltage-sensing domain was replaced with those from chicken, zebrafish, frog, mouse or human. We found that the chicken and zebrafish-based ArcLight exhibit faster kinetics, with a time constant (τ) less than 6 ms for a 100 mV depolarization. Although the response amplitude of these two probes (8-9%) is not as large as the Ciona-based ArcLight (~35%), they are better at reporting action potentials from cultured neurons at higher frequency. In contrast, probes based on frog, mouse and human voltage sensing domains were either slower than the Ciona-based ArcLight or had very small signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.