ArticleFrontiers in microbiology2013
Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases.Applied and environmental microbiology · 2025Article
- 1,2-DCA biodegradation potential of an aquifer assessed in situ and in aerobic and anaerobic microcosms.Environmental microbiome · 2024Article
- Heterologous Expression of ActiveApplied and environmental microbiology · 2022Article
- Microbiome Composition and Dynamics of a Reductive/Oxidative Bioelectrochemical System for Perchloroethylene Removal: Effect of the Feeding Composition.Frontiers in microbiology · 2022Article
- Molecular characterization of microbial communities in a peat-rich aquifer system contaminated with chlorinated aliphatic compounds.Environmental science and pollution research international · 2021Article
- Whole genome strategies and bioremediation insight into dehalogenase-producing bacteria.Molecular biology reports · 2021Review
- A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor.Environmental science and pollution research international · 2021Article
- The ever-expanding limits of enzyme catalysis and biodegradation: polyaromatic, polychlorinated, polyfluorinated, and polymeric compounds.The Biochemical journal · 2020Review
- Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms.Environmental science and pollution research international · 2020Article
- Hybrid Transcriptional Regulators for the Screening of Target DNA Motifs in Organohalide-Respiring Bacteria.Frontiers in microbiology · 2020Article
- Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater.Frontiers in microbiology · 2019Article
- Article
- PCB dechlorination hotspots and reductive dehalogenase genes in sediments from a contaminated wastewater lagoon.Environmental science and pollution research international · 2018Article
- Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds.Frontiers in microbiology · 2018Review
- Chlorinated Electron Acceptor Abundance Drives Selection ofFrontiers in microbiology · 2018Article
- Grape pomace compost harbors organohalide-respiring Dehalogenimonas species with novel reductive dehalogenase genes.The ISME journal · 2017Article
- Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.Chemical reviews · 2017Review
- Rapid Enrichment of Dehalococcoides-Like Bacteria by Partial Hydrophobic Separation.Applied and environmental microbiology · 2017Article
- Refined experimental annotation reveals conserved corrinoid autotrophy in chloroform-respiring Dehalobacter isolates.The ISME journal · 2017Article
- Comparative Single-Cell Genomics of Chloroflexi from the Okinawa Trough Deep-Subsurface Biosphere.Applied and environmental microbiology · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reductive dehalogenases are the critical enzymes for anaerobic organohalide respiration, a microbial metabolic process that has been harnessed for bioremediation efforts to resolve chlorinated solvent contamination in groundwater and is implicated in the global halogen cycle. Reductive dehalogenase sequence diversity is informative for the dechlorination potential of the site or enrichment culture. A suite of degenerate PCR primers targeting a comprehensive curated set of reductive dehalogenase genes was designed and applied to 12 DNA samples extracted from contaminated and pristine sites, as well as six enrichment cultures capable of reducing chlorinated compounds to non-toxic end-products. The amplified gene products from four environmental sites and two enrichment cultures were sequenced using Illumina HiSeq, and the reductive dehalogenase complement of each sample determined. The results indicate that the diversity of the reductive dehalogenase gene family is much deeper than is currently accounted for: one-third of the translated proteins have less than 70% pairwise amino acid identity to database sequences. Approximately 60% of the sequenced reductive dehalogenase genes were broadly distributed, being identified in four or more samples, and often in previously sequenced genomes as well. In contrast, 17% of the sequenced reductive dehalogenases were unique, present in only a single sample and bearing less than 90% pairwise amino acid identity to any previously identified proteins. Many of the broadly distributed reductive dehalogenases are uncharacterized in terms of their substrate specificity, making these intriguing targets for further biochemical experimentation. Finally, comparison of samples from a contaminated site and an enrichment culture derived from the same site 8 years prior allowed examination of the effect of the enrichment process.
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