ArticleThe Journal of biological chemistry2014
The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotrophic, nitrate-limiting conditions.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 140 citations in OpenAlex.
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- Toward Sustainability: Intensification of Light-Driven Whole Cell Biocatalysis.Chembiochem : a European journal of chemical biology · 2026Review
- Electrochemical Protection of Cyanobacterial Cells from Molecular Oxygen Enables Sustained PhotoHAngewandte Chemie (International ed. in English) · 2026Article
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- Chronic low-dose rate irradiation induces transient hormesis effect on cyanobacteriumiScience · 2025Article
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- CyAbrB2 is a nucleoid-associated protein ineLife · 2024Article
- A cellular selection identifies elongated flavodoxins that support electron transfer to sulfite reductase.Protein science : a publication of the Protein Society · 2023Article
- Photobiohydrogen Production and Strategies for HAdvances in biochemical engineering/biotechnology · 2023Article
- Regulation of organic acid and hydrogen production by NADH/NADFrontiers in microbiology · 2023Article
- Novel concepts and engineering strategies for heterologous expression of efficient hydrogenases in photosynthetic microorganisms.Frontiers in microbiology · 2023Review
- Evidence for Electron Transfer from the Bidirectional Hydrogenase to the Photosynthetic Complex I (NDH-1) in the CyanobacteriumMicroorganisms · 2022Article
- Genomic and Functional Variation of the ChlorophyllMicroorganisms · 2022Article
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- Dark fermentative hydrogen production and transcriptional analysis of genes involved in the unicellular halotolerant cyanobacteriumFrontiers in bioengineering and biotechnology · 2022Article
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- Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism inLife (Basel, Switzerland) · 2020Article
- Complete Genome Sequence of the Polysaccharide-Degrading Rumen Bacterium Pseudobutyrivibrio xylanivorans MA3014 Reveals an Incomplete Glycolytic Pathway.Genome biology and evolution · 2020Article
- Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.International journal of molecular sciences · 2020Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are able to use solar energy for the production of hydrogen. It is generally accepted that cyanobacterial NiFe-hydrogenases are reduced by NAD(P)H. This is in conflict with thermodynamic considerations, as the midpoint potentials of NAD(P)H do not suffice to support the measured hydrogen production under physiological conditions. We show that flavodoxin and ferredoxin directly reduce the bidirectional NiFe-hydrogenase of Synechocystis sp. PCC 6803 in vitro. A merodiploid ferredoxin-NADP reductase mutant produced correspondingly more photohydrogen. We furthermore found that the hydrogenase receives its electrons via pyruvate:flavodoxin/ferredoxin oxidoreductase (PFOR)-flavodoxin/ferredoxin under fermentative conditions, enabling the cells to gain ATP. These results strongly support that the bidirectional NiFe-hydrogenases in cyanobacteria function as electron sinks for low potential electrons from photosystem I and as a redox balancing device under fermentative conditions. However, the selective advantage of this enzyme is not known. No strong phenotype of mutants lacking the hydrogenase has been found. Because bidirectional hydrogenases are widespread in aquatic nutrient-rich environments that are capable of triggering phytoplankton blooms, we mimicked those conditions by growing cells in the presence of increased amounts of dissolved organic carbon and dissolved organic nitrogen. Under these conditions the hydrogenase was found to be essential. As these conditions close the two most important sinks for reduced flavodoxin/ferredoxin (CO2-fixation and nitrate reduction), this discovery further substantiates the connection between flavodoxin/ferredoxin and the NiFe-hydrogenase.
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