ArticleActa crystallographica. Section D, Biological crystallography2013
Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Attenuation of cancer proliferation by suppression of glypican-1 and its pleiotropic effects in neoplastic behavior.Oncotarget · 2023Article
- Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A temperature-controlled cold-gas humidifier and its application to protein crystals with the humid-air and glue-coating method.Journal of applied crystallography · 2019Article
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1-RanGTP-SPN1-Nup214 quaternary nuclear export complex.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Structural Aspects of N-Glycosylations and the C-terminal Region in Human Glypican-1.The Journal of biological chemistry · 2015Article
- Post-crystallization Improvement of RNA Crystal Diffraction Quality.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.Structure (London, England : 1993) · 2014Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.
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