ArticleActa crystallographica. Section D, Biological crystallography2013
Split green fluorescent protein as a modular binding partner for protein crystallization.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Controlling Megasynthetase Module-Module Interactions through β-Hairpin Docking Domain Engineering.ACS chemical biology · 2025Article
- Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.Scientific reports · 2025Article
- Dendritic atoh1a+ cells serve as Merkel cell precursors during skin development and regeneration.Development (Cambridge, England) · 2025Article
- Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assay.STAR protocols · 2025Article
- A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases.ACS catalysis · 2024Article
- Engineering highly stable variants of Corynactis californica green fluorescent proteins.Protein science : a publication of the Protein Society · 2024Article
- Nucleoplasmic lamin C rapidly accumulates at sites of nuclear envelope rupture with BAF and cGAS.The Journal of cell biology · 2022Article
- Ebola and Marburg virus VP35 coiled-coil validated as antiviral target by tripartite split-GFP complementation.iScience · 2022Article
- Engineering an efficient and bright split Corynactis californica green fluorescent protein.Scientific reports · 2021Article
- Commensal Neisseria cinerea impairs Neisseria meningitidis microcolony development and reduces pathogen colonisation of epithelial cells.PLoS pathogens · 2020Article
- Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology.International journal of molecular sciences · 2019Review
- Design of a chromogenic substrate for elastase based on split GFP system-Proof of concept for colour switch sensors.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.Annual review of biophysics · 2019Review
- Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals.Journal of the American Chemical Society · 2019Article
- Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.Communications biology · 2019Article
- Detecting In-Situ oligomerization of engineered STIM1 proteins by diffraction-limited optical imaging.PloS one · 2019Article
- Improved split fluorescent proteins for endogenous protein labeling.Nature communications · 2017Article
- Applying bimolecular fluorescence complementation to screen and purify aquaporin protein:protein complexes.Protein science : a publication of the Protein Society · 2016Article
- Versatile protein tagging in cells with split fluorescent protein.Nature communications · 2016Article
- Heterologous overexpression of sfCherry fluorescent protein inBiotechnology reports (Amsterdam, Netherlands) · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10-11) hairpin in complex with GFP(1-9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10-11) hairpin with a variety of GFP(1-9) mutants engineered for favorable crystallization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.