ArticleActa crystallographica. Section D, Biological crystallography2013
Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 61 citations in OpenAlex.
- Probing soft X-ray induced photoreduction of a model Mn-complex at cryogenic conditions.Journal of synchrotron radiation · 2025Article
- Energy Absorption and Beam Damage during Microfocus Synchrotron X-ray Diffraction.The journal of physical chemistry letters · 2024Article
- Does Acinetobacter calcoaceticus glucose dehydrogenase produce self-damaging H2O2?Bioscience reports · 2024Article
- Identifying and avoiding radiation damage in macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2024Article
- Serial macromolecular crystallography at ALBA Synchrotron Light Source.Journal of synchrotron radiation · 2022Article
- Quantifying and comparing radiation damage in the Protein Data Bank.Nature communications · 2022Article
- A SAXS-based approach to rationally evaluate radical scavengers - toward eliminating radiation damage in solution and crystallographic studies.Journal of synchrotron radiation · 2021Article
- Feasibility of cerium-doped LSO particles as a scintillator for x-ray induced optogenetics.Journal of neural engineering · 2021Article
- A Voltammetric Perspective of Multi-Electron and Proton Transfer in Protein Redox Chemistry: Insights From Computational Analysis ofFrontiers in chemistry · 2021Article
- Crystal structure of a conformational antibody that binds tau oligomers and inhibits pathological seeding by extracts from donors with Alzheimer's disease.The Journal of biological chemistry · 2020Article
- Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique.PloS one · 2020Article
- Article
- Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses.Journal of synchrotron radiation · 2019Article
- Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity.Scientific reports · 2019Article
- Efficacy of aldose reductase inhibitors is affected by oxidative stress induced under X-ray irradiation.Scientific reports · 2019Article
- Cys.sqlite: A Structured-Information Approach to the Comprehensive Analysis of Cysteine Disulfide Bonds in the Protein Databank.Journal of chemical information and modeling · 2019Article
- X-ray-induced sample damage at the Mn L-edge: a case study for soft X-ray spectroscopy of transition metal complexes in solution.Physical chemistry chemical physics : PCCP · 2018Article
- Article
- Real-space analysis of radiation-induced specific changes with independent component analysis.Journal of synchrotron radiation · 2018Article
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7 authors at 4 institutions in 2 countries.
Funding
Abstract
Electron paramagnetic resonance (EPR) and online UV-visible absorption microspectrophotometry with X-ray crystallography have been used in a complementary manner to follow X-ray-induced disulfide-bond cleavage. Online UV-visible spectroscopy showed that upon X-irradiation, disulfide radicalization appeared to saturate at an absorbed dose of approximately 0.5-0.8 MGy, in contrast to the saturating dose of ∼0.2 MGy observed using EPR at much lower dose rates. The observations suggest that a multi-track model involving product formation owing to the interaction of two separate tracks is a valid model for radiation damage in protein crystals. The saturation levels are remarkably consistent given the widely different experimental parameters and the range of total absorbed doses studied. The results indicate that even at the lowest doses used for structural investigations disulfide bonds are already radicalized. Multi-track considerations offer the first step in a comprehensive model of radiation damage that could potentially lead to a combined computational and experimental approach to identifying when damage is likely to be present, to quantitate it and to provide the ability to recover the native unperturbed structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.