ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2014
Development of DNA damage response signaling biomarkers using automated, quantitative image analysis.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Bioinformatics Analysis for Identifying Differentially Expressed MicroRNAs Derived from Plasma Exosomes Associated with Radiotherapy Resistance in Non-Small-Cell Lung Cancer.Applied bionics and biomechanics · 2022Article
- Immune Responses Vary in Preinvasive Colorectal Lesions by Tumor Location and Histology.Cancer prevention research (Philadelphia, Pa.) · 2021Article
- Brain Tumor Microenvironment and Angiogenesis in Melanoma Brain Metastases.Frontiers in oncology · 2020Article
- Intrinsic Astrocyte Heterogeneity Influences Tumor Growth in Glioma Mouse Models.Brain pathology (Zurich, Switzerland) · 2017Article
- Plasma miRNAs in predicting radiosensitivity in non-small cell lung cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Effective intra-S checkpoint responses to UVC in primary human melanocytes and melanoma cell lines.Pigment cell & melanoma research · 2016Article
- Treating Brain Tumor with Microbeam Radiation Generated by a Compact Carbon-Nanotube-Based Irradiator: Initial Radiation Efficacy Study.Radiation research · 2015Article
- IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Mechanisms of chromosomal instability in melanoma.Environmental and molecular mutagenesis · 2014Article
Corrections and comments
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Authors and funding
12 authors at 1 institution.
Funding
Abstract
The DNA damage response (DDR) coordinates DNA repair with cell cycle checkpoints to ameliorate or mitigate the pathological effects of DNA damage. Automated quantitative analysis (AQUA) and Tissue Studio are commercial technologies that use digitized immunofluorescence microscopy images to quantify antigen expression in defined tissue compartments. Because DDR is commonly activated in cancer and may reflect genetic instability within the lesion, a method to quantify DDR in cancer offers potential diagnostic and/or prognostic value. In this study, both AQUA and Tissue Studio algorithms were used to quantify the DDR in radiation-damaged skin fibroblasts, melanoma cell lines, moles, and primary and metastatic melanomas. Digital image analysis results for three markers of DDR (γH2AX, P-ATM, P-Chk2) correlated with immunoblot data for irradiated fibroblasts, whereas only γH2AX and P-Chk2 correlated with immunoblot data in melanoma cell lines. Melanoma cell lines displayed substantial variation in γH2AX and P-Chk2 expression, and P-Chk2 expression was significantly correlated with radioresistance. Moles, primary melanomas, and melanoma metastases in brain, lung and liver displayed substantial variation in γH2AX expression, similar to that observed in melanoma cell lines. Automated digital analysis of immunofluorescent images stained for DDR biomarkers may be useful for predicting tumor response to radiation and chemotherapy.
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Registered trials
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