ArticleJournal of the American Society of Nephrology : JASN2014
Two-photon microscopy reveals stationary podocytes in living zebrafish larvae.
Article in Journal of the American Society of Nephrology : JASN, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 49 citations in OpenAlex.
- Glomage: A Multimodal Platform for High-Content Morphological and RNA Profiling of Glomeruli in Zebrafish and Mouse Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Life of a Kidney Podocyte.Acta physiologica (Oxford, England) · 2025Review
- Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish.Journal of developmental biology · 2023Review
- Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.Nature communications · 2022Article
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- Role of Transient Receptor Potential Canonical Channel 6 (TRPC6) in Diabetic Kidney Disease by Regulating Podocyte Actin Cytoskeleton Rearrangement.Journal of diabetes research · 2020Review
- Adriamycin does not damage podocytes of zebrafish larvae.PloS one · 2020Article
- Novel fluorescence techniques to quantitate renal cell biology.Methods in cell biology · 2019Article
- BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function.Journal of cellular and molecular medicine · 2018Article
- The transcription factor Dach1 is essential for podocyte function.Journal of cellular and molecular medicine · 2018Article
- Novel Microscopic Techniques for Podocyte Research.Frontiers in endocrinology · 2018Review
- Advances in Renal Cell Imaging.Seminars in nephrology · 2018Review
- The Evolving Complexity of the Podocyte Cytoskeleton.Journal of the American Society of Nephrology : JASN · 2017Review
- A Heterologous Model of Thrombospondin Type 1 Domain-Containing 7A-Associated Membranous Nephropathy.Journal of the American Society of Nephrology : JASN · 2017Article
- Are podocytes motile?Pflugers Archiv : European journal of physiology · 2017Review
- New Insights into Podocyte Biology in Glomerular Health and Disease.Journal of the American Society of Nephrology : JASN · 2017Review
- Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae.Scientific reports · 2017Article
- Intravital and Kidney Slice Imaging of Podocyte Membrane Dynamics.Journal of the American Society of Nephrology : JASN · 2016Article
- Targeting the podocyte cytoskeleton: from pathogenesis to therapy in proteinuric kidney disease.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2016Review
- Intravital imaging in the kidney.Current opinion in nephrology and hypertension · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podocytes are an essential component of the glomerular filtration barrier and cover the outer aspect of glomerular capillaries. They form a complex actin-based cytoskeleton in vivo and show prominent motility in vitro, but whether podocytes are stationary or mobile in vivo is debated. To address this question, the pronephros of translucent zebrafish larvae (casper) expressing enhanced green fluorescent protein (eGFP) specifically in podocytes (wt1a:eGFP larvae) was observed by intravital two-photon microscopy over extended periods of time. Podocyte cell bodies and the interdigitating branching pattern of major processes could be resolved with a resolution of approximately 1 µm in the xy-plane. Time-lapse imaging of zebrafish larvae at 5-7 days after fertilization demonstrated that podocytes neither migrated nor changed the branching pattern of their major processes over a time period of up to 23 hours. In summary, we show by extended intravital two-photon microscopy that podocytes are stationary cells in the intact glomerulus of a translucent zebrafish with fluorescently-labeled podocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.