ArticleBMC biochemistry2013
Soybean peroxidase-mediated degradation of an azo dye- a detailed mechanistic study.
Article in BMC biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 77 citations in OpenAlex.
- Mechanistic insights and by-product analysis in sonocatalytic degradation of Orange-G dye molecule via potassium persulfate.Scientific reports · 2026Article
- Biodegradation and detoxification of malachite green dye by crude manganese peroxidase produced by Pseudomonas aeruginosa MF14446.1 and Enterobacter roggenkampii CP033800.1.Scientific reports · 2025Article
- Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide.Scientific reports · 2023Article
- Citrus limon peroxidase-assisted biocatalytic approach for biodegradation of reactive 1847 colfax blue P3R and 621 colfax blue R dyes.Bioprocess and biosystems engineering · 2023Article
- Microbial approaches for sustainable remediation of dye-contaminated wastewater: a review.Archives of microbiology · 2022Review
- Soybean Peroxidase Catalyzed Decoloration of Acid Azo Dyes.Journal of health & pollution · 2020Article
- Continuous degradation of Direct Red 23 by calcium pectate-bound Ziziphus mauritiana peroxidase: identification of metabolites and degradation routes.Environmental science and pollution research international · 2019Article
- Horseradish peroxidase-mediated decolourization of Orange II: modelling hydrogen peroxide utilization efficiency at different pH values.Environmental science and pollution research international · 2018Article
- Comparative Degradation of a Thiazole Pollutant by an Advanced Oxidation Process and an Enzymatic Approach.Biomolecules · 2017Article
- Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases.Biomolecules · 2017Article
- Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes.Environmental science and pollution research international · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeroxidases are emerging as an important class of enzymes that can be used for the efficient degradation of organic pollutants. However, detailed studies identifying the various intermediates produced and the mechanisms involved in the enzyme-mediated pollutant degradation are not widely published.
resultsIn the present study, the enzymatic degradation of an azo dye (Crystal Ponceau 6R, CP6R) was studied using commercially available soybean peroxidase (SBP) enzyme. Several operational parameters affecting the enzymatic degradation of dye were evaluated and optimized, such as initial dye concentration, H2O2 dosage, mediator amount and pH of the solution. Under optimized conditions, 40 ppm dye solution could be completely degraded in under one minute by SBP in the presence of H2O2 and a redox mediator. Dye degradation was also confirmed using HPLC and TOC analyses, which showed that most of the dye was being mineralized to CO2 in the process.
conclusionsDetailed analysis of metabolites, based on LC/MS results, showed that the enzyme-based degradation of the CP6R dye proceeded in two different reaction pathways- via symmetric azo bond cleavage as well as asymmetric azo bond breakage in the dye molecule. In addition, various critical transformative and oxidative steps such as deamination, desulfonation, keto-oxidation are explained on an electronic level. Furthermore, LC/MS/MS analyses confirmed that the end products in both pathways were small chain aliphatic carboxylic acids.
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