ArticleBMC genomics2013
Biosynthesis of the active compounds of Isatis indigotica based on transcriptome sequencing and metabolites profiling.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 71 citations in OpenAlex.
- Insights into the functions of MYB29 and MYB28.1 involved in glucosinolate biosynthesis from Isatis indigotica Fort.Functional & integrative genomics · 2026Article
- Genomic advances in orphan and underutilized Brassicaceae crops and their wild relatives.Frontiers in plant science · 2026Review
- The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense inActa pharmaceutica Sinica. B · 2024Article
- Mass Spectrometry Rearrangement Ions and Metabolic Pathway-Based Discovery of Indole Derivatives during the Aging Process inFoods (Basel, Switzerland) · 2023Article
- Light and Potassium Improve the Quality ofMolecules (Basel, Switzerland) · 2022Article
- Transcriptome analyses reveal the expression profile of genes related to lignan biosynthesis inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
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- Isatis indigotica: from (ethno) botany, biochemistry to synthetic biology.Molecular horticulture · 2021Review
- Integrative analyses of targeted metabolome and transcriptome of Isatidis Radix autotetraploids highlighted key polyploidization-responsive regulators.BMC genomics · 2021Article
- Tandem UGT71B5s Catalyze Lignan Glycosylation inFrontiers in plant science · 2021Article
- Genome-Wide Analysis of Light-Regulated Alternative Splicing inFrontiers in plant science · 2021Article
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- Mining genes associated with furanocoumarin biosynthesis in an endangered medicinal plant,Journal of genetics · 2020Article
- A chromosome-scale genome assembly ofHorticulture research · 2020Article
- Molecular cloning and metabolomic characterization of the 5-enolpyruvylshikimate-3-phosphate synthase gene from Baphicacanthus cusia.BMC plant biology · 2019Article
- Ultraviolet radiation for flavonoid augmentation inIndustrial crops and products · 2018Article
- Employing a biochemical protecting group for a sustainable indigo dyeing strategy.Nature chemical biology · 2018Article
- Remarkable enhancement of flavonoid production in a co-cultivation system ofIndustrial crops and products · 2018Article
- Recent advances and effective strategies in the discovery and applications of natural products.RSC advances · 2018Review
- Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIsatis indigotica is a widely used herb for the clinical treatment of colds, fever, and influenza in Traditional Chinese Medicine (TCM). Various structural classes of compounds have been identified as effective ingredients. However, little is known at genetics level about these active metabolites. In the present study, we performed de novo transcriptome sequencing for the first time to produce a comprehensive dataset of I. indigotica.
resultsA database of 36,367 unigenes (average length = 1,115.67 bases) was generated by performing transcriptome sequencing. Based on the gene annotation of the transcriptome, 104 unigenes were identified covering most of the catalytic steps in the general biosynthetic pathways of indole, terpenoid, and phenylpropanoid. Subsequently, the organ-specific expression patterns of the genes involved in these pathways, and their responses to methyl jasmonate (MeJA) induction, were investigated. Metabolites profile of effective phenylpropanoid showed accumulation pattern of secondary metabolites were mostly correlated with the transcription of their biosynthetic genes. According to the analysis of UDP-dependent glycosyltransferases (UGT) family, several flavonoids were indicated to exist in I. indigotica and further identified by metabolic profile using UPLC/Q-TOF. Moreover, applying transcriptome co-expression analysis, nine new, putative UGTs were suggested as flavonol glycosyltransferases and lignan glycosyltransferases.
conclusionsThis database provides a pool of candidate genes involved in biosynthesis of effective metabolites in I. indigotica. Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.
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