ArticleBioMed research international2013
Performance of glutamate dehydrogenase and triose phosphate isomerase genes in the analysis of genotypic variability of isolates of Giardia duodenalis from livestocks.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
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13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 601 citations in OpenAlex.
- Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals.PLoS neglected tropical diseases · 2025Pooled it
- Giardiasis as a neglected disease in Brazil: Systematic review of 20 years of publications.PLoS neglected tropical diseases · 2017Pooled it
- Giardia duodenalis and Entamoeba spp.: Coprological and antibody-based approaches across farm animals in Egypt.BMC veterinary research · 2026Article
- First report of prevalence and assemblage analysis of Giardia duodenalis in pigs from Guangxi Zhuang Autonomous Region, southern China.Parasite (Paris, France) · 2023Article
- Giardiasis in urban and rural Amazonas, Brazil is driven by zoonotic and cosmopolitan A and B assemblages.Memorias do Instituto Oswaldo Cruz · 2022Article
- Multilocus Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Epidemiology of Giardia duodenalis assemblages in Brazil: there is still a long way to go.Memorias do Instituto Oswaldo Cruz · 2021Review
- First description ofFood and waterborne parasitology · 2019Article
- Prevalence and multilocus genotyping of potentially zoonotic Giardia duodenalis in pigs in Shanghai, China.Parasitology · 2019Article
- First report of Giardia duodenalis genotypes in Zangxiang pigs from China.Parasitology research · 2019Article
- Presence of zoonotic Cryptosporidium scrofarum, Giardia duodenalis assemblage A and Enterocytozoon bieneusi genotypes in captive Eurasian wild boars (Sus scrofa) in China: potential for zoonotic transmission.Parasites & vectors · 2017Article
- The zoonotic potential of Giardia intestinalis assemblage E in rural settings.Parasitology research · 2016Article
- Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China.BMC veterinary research · 2014Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia duodenalis is a small intestinal protozoan parasite of several terrestrial vertebrates. This work aims to assess the genotypic variability of Giardia duodenalis isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) primers. Fecal samples from the three groups of animals were analyzed using the zinc sulphate centrifugal flotation technique. Out of 59 positive samples, 30 were from cattle, 26 from sheep and 3 from pigs. Cyst pellets were stored and submitted to PCR and nested-PCR reactions with gdh and tpi primers. Fragment amplification of gdh and tpi genes was observed in 25 (42.4%) and 36 (61.0%) samples, respectively. Regarding the sequencing, 24 sequences were obtained with gdh and 20 with tpi. For both genes, there was a prevalence of E specific species assemblage, although some isolates have been identified as A and B, by the tpi sequencing. This has also shown a larger number of heterogeneous sequences, which have been attribute to mixed infections between assemblages B and E. The largest variability of inter-assemblage associated to the frequency of heterogeneity provided by tpi sequencing reinforces the polymorphic nature of this gene and makes it an excellent target for studies on molecular epidemiology.
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