ArticleMolecular biology of the cell2014
Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in both plants and animals.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 90 citations in OpenAlex.
- Molecular choreography of vesicle trafficking: Exocyst-SNARE interplay in plant defense.Journal of advanced research · 2026Review
- Green nanomedicine for cancer therapy.Chinese medical journal · 2026Review
- Triple identity of medicinal plant-derived extracellular vesicles.Protoplasma · 2026Review
- Exosomes-mediated intercellular communication and their theragnostic potential against cancer and related disorders: a comprehensive review.Biotechnology letters · 2026Review
- Characterization of the OsEXO70 gene family and their response to salt stress in rice.BMC genomics · 2026Article
- Overexpression of EXO70E2 inGenes · 2026Article
- Structural and Functional Characterization of EXPO-Derived Extracellular Vesicles in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Molecular insights into the production of extracellular vesicles by plants.Plant physiology · 2026Article
- Stability Dynamics of Plant-Based Extracellular Vesicles Drug Delivery.Journal of xenobiotics · 2025Review
- The role of plant-derived extracellular vesicles in ameliorating chronic diseases.Molecular biology reports · 2025Review
- Advancements and Challenges of Plant-derived Extracellular Vesicles in Anti-Cancer Strategies and Drug Delivery.Current drug delivery · 2025Review
- Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid.Plant physiology · 2024Article
- Review
- Extracellular Vesicle Isolation and Mass Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Unlocking the Medicinal Potential of Plant-Derived Extracellular Vesicles: current Progress and Future Perspectives.International journal of nanomedicine · 2024Review
- Plant Extracellular Vesicles: Current Landscape and Future Directions.Plants (Basel, Switzerland) · 2023Review
- Plant Exosome-like Nanovesicles and Their Role in the Innovative Delivery of RNA Therapeutics.Biomedicines · 2023Review
- Extracellular vesicles: cross-organismal RNA trafficking in plants, microbes, and mammalian cells.Extracellular vesicles and circulating nucleic acids · 2023Article
- Vesicle trafficking in rice: too little is known.Frontiers in plant science · 2023Review
- Plant and mammalian-derived extracellular vesicles: a new therapeutic approach for the future.Frontiers in bioengineering and biotechnology · 2023Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to a single copy of Exo70 in yeast and mammals, the Arabidopsis genome contains 23 paralogues of Exo70 (AtExo70). Using AtExo70E2 and its GFP fusion as probes, we recently identified a novel double-membrane organelle termed exocyst-positive organelle (EXPO) that mediates an unconventional protein secretion in plant cells. Here we further demonstrate that AtExo70E2 is essential for exocyst subunit recruitment and for EXPO formation in both plants and animals. By performing transient expression in Arabidopsis protoplasts, we established that a number of exocyst subunits (especially the members of the Sec family) are unable to be recruited to EXPO in the absence of AtExo70E2. The paralogue AtExo70A1 is unable to substitute for AtExo70E2 in this regard. Fluorescence resonance energy transfer assay and bimolecular fluorescence complementation analyses confirm the interaction between AtExo70E2 and Sec6 and Sec10. AtExo70E2, but not its yeast counterpart, is also capable of inducing EXPO formation in an animal cell line (HEK293A cells). Electron microscopy confirms the presence of double-membraned, EXPO-like structures in HEK293A cells expressing AtExo70E2. Inversely, neither human nor yeast Exo70 homologues cause the formation of EXPO in Arabidopsis protoplasts. These results point to a specific and crucial role for AtExo70E2 in EXPO formation.
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