ArticleObesity (Silver Spring, Md.)2014
Prolonged efficiency of siRNA-mediated gene silencing in primary cultures of human preadipocytes and adipocytes.
Article in Obesity (Silver Spring, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05275608 (Effects of Very Low Calorie Ketogenic Diet on Microbiota, Adipose Tissue and Immunitary Regulation), which is not on this map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effects of Very Low Calorie Ketogenic Diet on Microbiota, Adipose Tissue and Immunitary Regulation: Pilot Study on Patients with Metabolic Syndrome
Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- The integrin beta1 modulator Tirofiban prevents adipogenesis and obesity by the overexpression of integrin-linked kinase: a pre-clinical approach in vitro and in vivo.Cell & bioscience · 2022Article
- PIEZO1 mechanoreceptor activation reduces adipogenesis in perivascular adipose tissue preadipocytes.Frontiers in endocrinology · 2022Article
- Further evidence supporting a potential role for ADH1B in obesity.Scientific reports · 2021Article
- CIDEA Transcriptionally Regulates UCP1 for Britening and Thermogenesis in Human Fat Cells.iScience · 2019Article
- Impaired Glucocorticoid Suppression of TGFβ Signaling in Human Omental Adipose Tissues Limits Adipogenesis and May Promote Fibrosis.Diabetes · 2019Article
- Growth hormone acts along the PPARγ-FSP27 axis to stimulate lipolysis in human adipocytes.American journal of physiology. Endocrinology and metabolism · 2019Article
- Dendritic peptide bolaamphiphiles for siRNA delivery to primary adipocytes.Biomaterials · 2018Article
- Low expression of the GILZ may contribute to adipose inflammation and altered adipokine production in human obesity.Journal of lipid research · 2016Article
- Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review.International journal of molecular sciences · 2016Review
- Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.The Journal of biological chemistry · 2014Article
- An siRNA-based method for efficient silencing of gene expression in mature brown adipocytes.AdipocyteArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
objectivePrimary human preadipocytes and differentiated adipocytes in culture are valuable cell culture systems to study adipogenesis and adipose function in relation to human adipose biology. To use these systems for mechanistic studies, siRNA-mediated knockdown of genes for its effectiveness was studied.
methodsMethods were developed to effectively deliver siRNA for gene silencing in primary preadipocytes isolated from human subcutaneous adipose tissue and newly differentiated adipocytes. Expression level of genes and proteins was measured using quantitative RT-PCR and western blotting. Lipid droplet morphology was observed using microscopy, and glycerol release was quantified as a measure of lipolysis.
resultssiRNA-mediated knockdown of genes in primary human preadipocytes resulted in prolonged silencing effects, suppressing genes throughout the process of their differentiation. In newly differentiated adipocytes, siRNA-mediated gene knockdown allowed proteins to stay depleted for at least 5 days. It was possible to re-express a protein after its siRNA-mediated depletion. Importantly, siRNA transfected human adipocytes remained metabolically active, responding to β-adrenergic stimulation to increase lipolysis.
conclusionsOur study describes the methods of gene silencing in primary cultures of human preadipocytes and adipocytes and their prolonged effectiveness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.