ArticleGenome biology and evolution2013
Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig).
Article in Genome biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 122 citations in OpenAlex.
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- Expanded gene and taxon sampling of diplomonads shows multiple switches to parasitic and free-living lifestyle.BMC biology · 2024Article
- Insights into Peptidyl-ProlylPathogens (Basel, Switzerland) · 2024Review
- Comparative genomics of Giardia duodenalis sub-assemblage AI beaver (Be-2) and human (WB-C6) strains show remarkable homozygosity, sequence similarity, and conservation of VSP genes.Scientific reports · 2024Article
- Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis.Genetics · 2024Review
- Genomic survey maps differences in the molecular complement of vesicle formation machinery between Giardia intestinalis assemblages.PLoS neglected tropical diseases · 2023Article
- Spliceosomal introns in the diplomonad parasiteMicrobial genomics · 2023Article
- Highly contiguous genomes of human clinical isolates ofMicrobial genomics · 2023Article
- Variation in haplotypes in single cysts of assemblages C and D, but not of assemblage E of Giardia duodenalis.BMC microbiology · 2022Article
- Genomic comparisons confirmFrontiers in cellular and infection microbiology · 2022Article
- A hybrid sequencing and assembly strategy for generating culture freeCurrent research in microbial sciences · 2022Article
- Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.Nature communications · 2021Article
- Article
- Suitability of current typing procedures to identify epidemiologically linked human Giardia duodenalis isolates.PLoS neglected tropical diseases · 2021Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Giardia lamblia (syn G. intestinalis, G. duodenalis) is the most common pathogenic intestinal parasite of humans worldwide and is a frequent cause of endemic and epidemic diarrhea. G. lamblia is divided into eight genotypes (A-H) which infect a wide range of mammals and humans, but human infections are caused by Genotypes A and B. To unambiguously determine the relationship among genotypes, we sequenced GS and DH (Genotypes B and A2) to high depth coverage and compared the assemblies with the nearly completed WB genome and draft sequencing surveys of Genotypes E (P15; pig isolate) and B (GS; human isolate). Our results identified DH as the smallest Giardia genome sequenced to date, while GS is the largest. Our open reading frame analyses and phylogenetic analyses showed that GS was more distant from the other three genomes than any of the other three were from each other. Whole-genome comparisons of DH_A2 and GS_B with the optically mapped WB_A1 demonstrated substantial synteny across all five chromosomes but also included a number of rearrangements, inversions, and chromosomal translocations that were more common toward the chromosome ends. However, the WB_A1/GS_B alignment demonstrated only about 70% sequence identity across the syntenic regions. Our findings add to information presented in previous reports suggesting that GS is a different species of Giardia as supported by the degree of genomic diversity, coding capacity, heterozygosity, phylogenetic distance, and known biological differences from WB_A1 and other G. lamblia genotypes.
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