ArticleDevelopment (Cambridge, England)2014
Ion channels contribute to the regulation of cell sheet forces during Drosophila dorsal closure.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 61 citations in OpenAlex.
- Mechanostimulation of neuroendocrine cells and their clinical relevance.Molecular and cellular biochemistry · 2026Review
- TRPA1 for Butterfly Eyespot Formation.International journal of molecular sciences · 2026Article
- A steady-state pool of calcium-dependent actin is maintained by Homer and controls epithelial mechanosensation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Deciphering the Calcium Code: A Review of Calcium Activity Analysis Methods Employed to Identify Meaningful Activity in Early Neural Development.Biomolecules · 2024Review
- Superresolution microscopy reveals actomyosin dynamics in medioapical arrays.Molecular biology of the cell · 2022Article
- Article
- Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.The Journal of cell biology · 2022Article
- Optochemical Control of Cell Contractility in Drosophila Embryos.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.EMBO reports · 2021Article
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Article
- A simple mechanochemical model for calcium signalling in embryonic epithelial cells.Journal of mathematical biology · 2019Article
- Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch.Nature communications · 2019Article
- Dynamics of PAR Proteins Explain the Oscillation and Ratcheting Mechanisms in Dorsal Closure.Biophysical journal · 2018Article
- Secretory pathway calcium ATPase 1 (SPCA1) controls mouse neural tube closure by regulating cytoskeletal dynamics.Development (Cambridge, England) · 2018Article
- Mathematical models of dorsal closure.Progress in biophysics and molecular biology · 2018Review
- Identifying Genetic Players in Cell Sheet Morphogenesis Using aG3 (Bethesda, Md.) · 2018Article
- Electromechanics and Volume Dynamics in Nonexcitable Tissue Cells.Biophysical journal · 2018Article
- Cell Sheet Morphogenesis: Dorsal Closure in Drosophila melanogaster as a Model System.Annual review of cell and developmental biology · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate that ion channels contribute to the regulation of dorsal closure in Drosophila, a model system for cell sheet morphogenesis. We find that Ca(2+) is sufficient to cause cell contraction in dorsal closure tissues, as UV-mediated release of caged Ca(2+) leads to cell contraction. Furthermore, endogenous Ca(2+) fluxes correlate with cell contraction in the amnioserosa during closure, whereas the chelation of Ca(2+) slows closure. Microinjection of high concentrations of the peptide GsMTx4, which is a specific modulator of mechanically gated ion channel function, causes increases in cytoplasmic free Ca(2+) and actomyosin contractility and, in the long term, blocks closure in a dose-dependent manner. We identify two channel subunits, ripped pocket and dtrpA1 (TrpA1), that play a role in closure and other morphogenetic events. Blocking channels leads to defects in force generation via failure of actomyosin structures, and impairs the ability of tissues to regulate forces in response to laser microsurgery. Our results point to a key role for ion channels in closure, and suggest a mechanism for the coordination of force-producing cell behaviors across the embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.