ArticleNature communications2013
Mechanisms of molecular transport through the urea channel of Helicobacter pylori.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.Current protocols · 2026Article
- Yeast Complementation Assays Demonstrating the Importance of the Affinity Tag Position in Membrane Protein Purification, as Exemplified bySmall science · 2025Article
- Carbon dioxide regulatesInfection and immunity · 2025Article
- Enhanced Site-Specific Fluorescent Labeling of Membrane Proteins Using Native Nanodiscs.Biomolecules · 2025Article
- New Directions inInfection and drug resistance · 2025Review
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Urea Transport Mediated by Membrane Proteins of Non-urea-Transporters.Sub-cellular biochemistry · 2025Review
- Structural insights into the mechanisms of urea permeation and distinct inhibition modes of urea transporters.Nature communications · 2024Article
- Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions.Biomolecules · 2022Article
- Cryo-EM for Small Molecules.Current protocols · 2022Review
- Biomimetic Artificial Proton Channels.Biomolecules · 2022Review
- Prediction and Inferred Evolution of Acid Tolerance Genes in the Biotechnologically ImportantFrontiers in microbiology · 2022Article
- Free energy and kinetics of cAMP permeation through connexin26 via applied voltage and milestoning.Biophysical journal · 2021Article
- Water in Nanopores and Biological Channels: A Molecular Simulation Perspective.Chemical reviews · 2020Review
- Urea-aromatic interactions in biology.Biophysical reviews · 2020Review
- How Melittin Inserts into Cell Membrane: Conformational Changes, Inter-Peptide Cooperation, and Disturbance on the Membrane.Molecules (Basel, Switzerland) · 2019Review
- pH-dependent gating mechanism of theScience advances · 2019Article
- Protection AgainstFrontiers in immunology · 2018Article
- Sodium butyrate mitigates in vitro ammonia generation in cecal content of laying hens.Environmental science and pollution research international · 2016Article
- An Integrated Framework Advancing Membrane Protein Modeling and Design.PLoS computational biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
Helicobacter pylori survival in acidic environments relies on cytoplasmic hydrolysis of gastric urea into ammonia and carbon dioxide, which buffer the pathogen's periplasm. Urea uptake is greatly enhanced and regulated by HpUreI, a proton-gated inner membrane channel protein essential for gastric survival of H. pylori. The crystal structure of HpUreI describes a static snapshot of the channel with two constriction sites near the center of the bilayer that are too narrow to allow passage of urea or even water. Here we describe the urea transport mechanism at atomic resolution, revealed by unrestrained microsecond equilibrium molecular dynamics simulations of the hexameric channel assembly. Two consecutive constrictions open to allow conduction of urea, which is guided through the channel by interplay between conserved residues that determine proton rejection and solute selectivity. Remarkably, HpUreI conducts water at rates equivalent to aquaporins, which might be essential for efficient transport of urea at small concentration gradients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.