ArticleNature communications2013
KaiC intersubunit communication facilitates robustness of circadian rhythms in cyanobacteria.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Effect of pH on the cyanobacterial circadian oscillator in vitro.Communications biology · 2025Article
- Review
- Photomorphogenesis of Myxococcus macrosporus: new insights for light-regulation of cell development.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Roles for theJournal of biological rhythms · 2023Article
- Role of the reaction-structure coupling in temperature compensation of the KaiABC circadian rhythm.PLoS computational biology · 2022Article
- Regulation mechanisms of the dual ATPase in KaiC.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multimeric structure enables the acceleration of KaiB-KaiC complex formation induced by ADP/ATP exchange inhibition.PLoS computational biology · 2022Article
- Translating around the clock: Multi-level regulation of post-transcriptional processes by the circadian clock.Cellular signalling · 2021Review
- Mechanism of autonomous synchronization of the circadian KaiABC rhythm.Scientific reports · 2021Article
- Monitoring Protein-Protein Interactions in the Cyanobacterial Circadian Clock in Real Time via Electron Paramagnetic Resonance Spectroscopy.Biochemistry · 2020Article
- Polyamines Disrupt the KaiABC Oscillator by Inducing Protein Denaturation.Molecules (Basel, Switzerland) · 2019Article
- Molecular dynamics simulations of nucleotide release from the circadian clock protein KaiC reveal atomic-resolution functional insights.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Non-transcriptional processes in circadian rhythm generation.Current opinion in physiology · 2018Review
- Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time.Nature communications · 2018Article
- Single-molecular and ensemble-level oscillations of cyanobacterial circadian clock.Biophysics and physicobiology · 2018Article
- Role of ATP Hydrolysis in Cyanobacterial Circadian Oscillator.Scientific reports · 2017Article
- Minimal tool set for a prokaryotic circadian clock.BMC evolutionary biology · 2017Article
- Period Robustness and Entrainability of the Kai System to Changing Nucleotide Concentrations.Biophysical journal · 2017Article
- Low temperature nullifies the circadian clock in cyanobacteria through Hopf bifurcation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cyanobacterial circadian clock is the only model clock to have been reconstituted in vitro. KaiC, the central clock component, is a homohexameric ATPase with autokinase and autophosphatase activities. Changes in phosphorylation state have been proposed to switch KaiC's activity between autokinase and autophosphatase. Here we analyse the molecular mechanism underlying the regulation of KaiC's activity, in the context of its hexameric structure. We reconstitute KaiC hexamers containing different variant protomers, and measure their autophosphatase and autokinase activities. We identify two types of regulatory mechanisms with distinct functions. First, local interactions between adjacent phosphorylation sites regulate KaiC's activities, coupling the ATPase and nucleotide-binding states at subunit interfaces of the CII domain. Second, the phosphorylation states of the protomers affect the overall activity of KaiC hexamers via intersubunit communication. Our findings indicate that intra-hexameric interactions play an important role in sustaining robust circadian rhythmicity.
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