ArticleNature communications2013
Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
109 citing papers in PubMed, 1 synthesis or guideline pooled it, 271 citations in OpenAlex.
- Pooled it
- Genomic and chemical analyses of 713 marine biofilm-derived bacterial strains.Applied and environmental microbiology · 2026Article
- A comprehensive review on antifungal compounds and biogenic nanoparticles from marine actinobacteria against opportunistic fungal infections.Folia microbiologica · 2026Review
- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Construction and Diversification of Natural Product Biosynthetic Gene Clusters at High Efficiency and Accuracy.ACS synthetic biology · 2025Article
- Integrated genome mining and molecular networking uncover the biosynthetic potential of a novel mangrove-derived strain Streptomyces sp. B1866.BMC genomics · 2025Article
- Genomic insights into biosynthesis and adaptation in the bioactive marine bacterium Streptomyces albidoflavus VIP-1 from the Red Sea.BMC microbiology · 2025Article
- Strategies for Actinobacteria Isolation, Cultivation, and Metabolite Production that Are Biologically Important.ACS omega · 2025Review
- Medical Potential of Insect Symbionts.Insects · 2025Review
- Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces.Science China. Life sciences · 2025Article
- A guide to genome mining and genetic manipulation of biosynthetic gene clusters in Streptomyces.Journal of microbiology (Seoul, Korea) · 2025Article
- Expanding Polycyclic Tetramate Macrolactam (PoTeM) Core Structure Diversity by Chemo-Enzymatic Synthesis and Bioengineering.Angewandte Chemie (International ed. in English) · 2025Article
- Metabolomic and genomic insights into Micromonospora carbonacea subsp. caeruleus for anti-colorectal compound.Applied microbiology and biotechnology · 2025Article
- Diversity of secondary metabolites from marine Streptomyces with potential anti-tubercular activity: a review.Archives of microbiology · 2025Review
- A plug-and-play system for polycyclic tetramate macrolactam production and functionalization.Microbial cell factories · 2025Article
- An Overview of Lsr2 Repressor Effect inMicroorganisms · 2024Article
- Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces.Nature communications · 2024Article
- Rational construction of genome-minimizedSynthetic and systems biotechnology · 2024Article
- Analysis of Co-localized Biosynthetic Gene Clusters Identifies a Membrane-Permeabilizing Natural Product.Journal of natural products · 2024Article
- Critical analysis of polycyclic tetramate macrolactam biosynthetic gene cluster phylogeny and functional diversity.Applied and environmental microbiology · 2024Article
49 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Polycyclic tetramate macrolactams (PTMs) are a widely distributed class of natural products with important biological activities. However, many of these PTMs have not been characterized. Here we apply a plug-and-play synthetic biology strategy to activate a cryptic PTM biosynthetic gene cluster SGR810-815 from Streptomyces griseus and discover three new PTMs. This gene cluster is highly conserved in phylogenetically diverse bacterial strains and contains an unusual hybrid polyketide synthase-nonribosomal peptide synthetase, which resembles iterative polyketide synthases known in fungi. To further characterize this gene cluster, we use the same synthetic biology approach to create a series of gene deletion constructs and elucidate the biosynthetic steps for the formation of the polycyclic system. The strategy we employ bypasses the traditional laborious processes to elicit gene cluster expression and should be generally applicable to many other silent or cryptic gene clusters for discovery and characterization of new natural products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.