ArticleNature communications2013
Essential regulation of cell bioenergetics in Trypanosoma brucei by the mitochondrial calcium uniporter.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 122 citations in OpenAlex.
- Review
- The discovery of the Sph-gated plasma membrane CaBiophysical reviews · 2025Review
- Poly(A)-binding protein is an ataxin-2 chaperone that regulates biomolecular condensates.Molecular cell · 2023Article
- A spatially resolved elemental nanodomain organization within acidocalcisomes inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Autophagy in protists and their hosts: When, how and why?Autophagy reports · 2023Article
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- MICU1 and MICU2 potentiation of CaCell calcium · 2022Article
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- Mitochondrial CaAntioxidants & redox signaling · 2022Review
- Mitochondrial calcium exchange in physiology and disease.Physiological reviews · 2022Review
- CaCells · 2022Review
- An aromatic imidazoline derived from chloroquinoline triggers cell cycle arrest and inhibits with high selectivity the Trypanosoma cruzi mammalian host-cells infection.PLoS neglected tropical diseases · 2021Article
- Trypanosoma cruzi Letm1 is involved in mitochondrial CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- From the Identification to the Dissection of the Physiological Role of the Mitochondrial Calcium Uniporter: An Ongoing Story.Biomolecules · 2021Review
- Article
- The IPBiochimica et biophysica acta. Molecular cell research · 2021Review
- Intracellular CaInternational journal of molecular sciences · 2021Review
- Mitochondrial CaInternational review of cell and molecular biology · 2021Review
- Trypanosomatid selenophosphate synthetase structure, function and interaction with selenocysteine lyase.PLoS neglected tropical diseases · 2020Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Mechanisms of regulation of mitochondrial metabolism in trypanosomes are not completely understood. Here we present evidence that the Trypanosoma brucei mitochondrial calcium uniporter (TbMCU) is essential for the regulation of mitochondrial bioenergetics, autophagy and cell death, even in the bloodstream forms that are devoid of a functional respiratory chain and oxidative phosphorylation. Localization studies reveal its co-localization with MitoTracker staining. TbMCU overexpression increases mitochondrial Ca(2+) accumulation in intact and permeabilized trypanosomes, generates excessive mitochondrial reactive oxygen species (ROS) and sensitizes them to apoptotic stimuli. Ablation of TbMCU in RNAi or conditional knockout trypanosomes reduces mitochondrial Ca(2+) uptake without affecting their membrane potential, increases the AMP/ATP ratio, stimulates autophagosome formation and produces marked defects in growth in vitro and infectivity in mice, revealing its essentiality in these parasites. The requirement of TbMCU for proline and pyruvate metabolism in procyclic and bloodstream forms, respectively, reveals its role in regulation of mitochondrial bioenergetics.
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Registered trials
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