ArticleScientific reports2013
A non-surgical approach for male germ cell mediated gene transmission through transgenesis.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis.Molecular biology reports · 2026Article
- A stable and potent buffalo EF1α1 promoter for robust gene expression in mammalian systems.Transgenic research · 2026Article
- Article
- A Simple and Safe Protocol for Intra-Testicular Gene Delivery in Neonatal Mice Using a Convenient Isoflurane-Based Anesthesia System.Biotech (Basel (Switzerland)) · 2025Article
- Article
- Follicle-stimulating hormone-mediated decline in miR-92a-3p expression in pubertal mice Sertoli cells is crucial for germ cell differentiation and fertility.Cellular and molecular life sciences : CMLS · 2022Article
- Declining levels of miR-382-3p at puberty trigger the onset of spermatogenesis.Molecular therapy. Nucleic acids · 2021Article
- Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells.Scientific reports · 2018Article
- An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation.Molecular therapy. Nucleic acids · 2016Article
- Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells.Molecular therapy. Methods & clinical development · 2016Article
- An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo.Journal of animal science and biotechnology · 2015Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjection of foreign DNA in male pronucleus by in-vitro embryo manipulation is difficult but remains the method of choice for generating transgenic animals. Other procedures, including retroviral and embryonic stem cell mediated transgenesis are equally complicated and have limitations. Although our previously reported technique of testicular transgenesis circumvented several limitations, it involved many steps, including surgery and hemicastration, which carried risk of infection and impotency. We improved this technique further, into a two step non-surgical electroporation procedure, for making transgenic mice. In this approach, transgene was delivered inside both testes by injection and modified parameters of electroporation were used for in-vivo gene integration in germ cells. Using variety of constructs, germ cell integration of the gene and its transmission in progeny was confirmed by PCR, slot blot and immunohistochemical analysis. This improved technique is efficient, requires substantially less time and can be easily adopted by various biomedical researchers.
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Registered trials
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