ArticleBMC complementary and alternative medicine2013
Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts.
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- Organic extract ofOncology letters · 2022Article
- Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2022Article
- Ubiquitousness ofMarine drugs · 2021Article
- A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and GScientific reports · 2020Article
- Deep Hypersaline Anoxic Basins as Untapped Reservoir of Polyextremophilic Prokaryotes of Biotechnological Interest.Marine drugs · 2020Review
- Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine.Marine drugs · 2019Review
- Marine Natural Products: A Source of Novel Anticancer Drugs.Marine drugs · 2019Review
- Mining the deep Red-Sea brine pool microbial community for anticancer therapeutics.BMC complementary and alternative medicine · 2019Article
- Marine anticancer drugs and their relevant targets: a treasure from the ocean.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2019Review
- Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool.Microbial cell factories · 2019Article
- Anticancer properties of chitosan against osteosarcoma, breast cancer and cervical cancer cell lines.Caspian journal of internal medicine · 2019Article
- Supernatant Metabolites from Halophilic Archaea to Reduce Tumorigenesis in Prostate CancerIranian journal of pharmaceutical research : IJPR · 2019Article
- Genome sequence analysis ofBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Identification of Halophilic Microbes in Lung Fibrotic Tissue by Oligotyping.Frontiers in microbiology · 2018Article
- Improved detection of gene-microbe interactions in the mouse skin microbiota using high-resolution QTL mapping of 16S rRNA transcripts.Microbiome · 2017Article
- Building a bio-based industry in the Middle East through harnessing the potential of the Red Sea biodiversity.Applied microbiology and biotechnology · 2017Review
- Broad targeting of resistance to apoptosis in cancer.Seminars in cancer biology · 2015Review
- Gecko proteins induce the apoptosis of bladder cancer 5637 cells by inhibiting Akt and activating the intrinsic caspase cascade.BMB reports · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMarine microorganisms are considered to be an important source of bioactive molecules against various diseases and have great potential to increase the number of lead molecules in clinical trials. Progress in novel microbial culturing techniques as well as greater accessibility to unique oceanic habitats has placed the marine environment as a new frontier in the field of natural product drug discovery.
methodsA total of 24 microbial extracts from deep-sea brine pools in the Red Sea have been evaluated for their anticancer potential against three human cancer cell lines. Downstream analysis of these six most potent extracts was done using various biological assays, such as Caspase-3/7 activity, mitochondrial membrane potential (MMP), PARP-1 cleavage and expression of γH2Ax, Caspase-8 and -9 using western blotting.
resultsIn general, most of the microbial extracts were found to be cytotoxic against one or more cancer cell lines with cell line specific activities. Out of the 13 most active microbial extracts, six extracts were able to induce significantly higher apoptosis (>70%) in cancer cells. Mechanism level studies revealed that extracts from Chromohalobacter salexigens (P3-86A and P3-86B(2)) followed the sequence of events of apoptotic pathway involving MMP disruption, caspase-3/7 activity, caspase-8 cleavage, PARP-1 cleavage and Phosphatidylserine (PS) exposure, whereas another Chromohalobacter salexigens extract (K30) induced caspase-9 mediated apoptosis. The extracts from Halomonas meridiana (P3-37B), Chromohalobacter israelensis (K18) and Idiomarina loihiensis (P3-37C) were unable to induce any change in MMP in HeLa cancer cells, and thus suggested mitochondria-independent apoptosis induction. However, further detection of a PARP-1 cleavage product, and the observed changes in caspase-8 and -9 suggested the involvement of caspase-mediated apoptotic pathways.
conclusionAltogether, the study offers novel findings regarding the anticancer potential of several halophilic bacterial species inhabiting the Red Sea (at the depth of 1500-2500 m), which constitute valuable candidates for further isolation and characterization of bioactive molecules.
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