ArticleThe ISME journal2014
Marine viruses, a genetic reservoir revealed by targeted viromics.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed.
- Gut virome dynamics: from commensal to critical player in health and disease.Nature reviews. Gastroenterology & hepatology · 2026Review
- Biogeography of Virioplankton Abundance and Subcluster Patterns in the Northwest Pacific: A Large-Scale Perspective.MicrobiologyOpen · 2025Article
- Unexpected diversity and ecological significance of uncultivable large virus-like particles in aquatic environments.ISME communications · 2025Article
- Metavirome Insights into the Diversity and Potential Pathogenic Infection ofPathogens (Basel, Switzerland) · 2024Article
- Giant virus biology and diversity in the era of genome-resolved metagenomics.Nature reviews. Microbiology · 2022Review
- Article
- Informative Regions In Viral Genomes.Viruses · 2021Article
- An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry.Viruses · 2021Article
- Viral-Mediated Microbe Mortality Modulated by Ocean Acidification and Eutrophication: Consequences for the Carbon Fluxes Through the Microbial Food Web.Frontiers in microbiology · 2021Article
- Virioplankton distribution in the tropical western Pacific Ocean in the vicinity of a seamount.MicrobiologyOpen · 2020Article
- Weekly variations of viruses and heterotrophic nanoflagellates and their potential impact on bacterioplankton in shallow waters of the central Red Sea.FEMS microbiology ecology · 2020Article
- Shining Light on Human Gut Bacteriophages.Frontiers in cellular and infection microbiology · 2020Review
- Impacts of Freshwater and Seawater Mixing on the Production and Decay of Virioplankton in a Subtropical Estuary.Microbial ecology · 2019Article
- Review
- Viral Communities in the Global Deep Ocean Conveyor Belt Assessed by Targeted Viromics.Frontiers in microbiology · 2019Article
- High-throughput isolation of giant viruses using high-content screening.Communications biology · 2019Article
- Fifteen Marseilleviruses Newly Isolated From Three Water Samples in Japan Reveal Local Diversity ofFrontiers in microbiology · 2019Article
- Hidden diversity of soil giant viruses.Nature communications · 2018Article
- Flow virometry as a tool to study viruses.Methods (San Diego, Calif.) · 2018Review
- An Insight into Phage Diversity at Environmental Habitats using Comparative Metagenomics Approach.Current microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metagenomics has opened new windows on investigating viral diversity and functions. Viromic studies typically require large sample volumes and filtration through 0.2 μm pore-size filters, consequently excluding or under-sampling tailed and very large viruses. We have optimized a targeted viromic approach that employs fluorescence-activated sorting and whole genome amplification to produce dsDNA-enriched libraries from discrete viral populations from a 1-ml water sample. Using this approach on an environmental sample from the Patagonian Shelf, we produced three distinct libraries. One of the virus libraries was dominated (79.65% of sequences with known viral homology) by giant viruses from the Mimiviridae and Phycodnaviridae families, while the two other viromes were dominated by smaller phycodnaviruses, cyanophages and other bacteriophages. The estimated genotypic richness and diversity in our sorted viromes, with 52-163 estimated genotypes, was much lower than in previous virome reports. Fragment recruitment of metagenome reads to selected reference viral genomes yields high genome coverage, suggesting little amplification and sequencing bias against some genomic regions. These results underscore the value of our approach as an effective way to target and investigate specific virus groups. In particular, it will help reveal the diversity and abundance of giant viruses in marine ecosystems.
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