Evidence map›Paper›PMID 24304553›Full record

ArticleBMC genetics2013

Description of durum wheat linkage map and comparative sequence analysis of wheat mapped DArT markers with rice and Brachypodium genomes.

Pasqualina Colasuonno, Mastrangelo Anna Maria, Antonio Blanco, Agata Gadaleta

Abstract readComparative Study
In one paragraph

Article in BMC genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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5 citing papers in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Pasqualina Colasuonno
Mastrangelo Anna Maria
Antonio Blanco
Agata GadaletaDepartment of Soil, Plant and Food Sciences, University of Bari "Aldo Moro", Via Amendola 165/A, Bari 70126, Italy. agata.gadaleta@uniba.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe importance of wheat to the world economy, together with progresses in high-throughput next-generation DNA sequencing, have accelerated initiatives of genetic research for wheat improvement. The availability of high density linkage maps is crucial to identify genotype-phenotype associations, but also for anchoring BAC contigs to genetic maps, a strategy followed for sequencing the wheat genome.

resultsHere we report a genetic linkage map in a durum wheat segregating population and the study of mapped DArT markers. The linkage map consists of 126 gSSR, 31 EST-SSR and 351 DArT markers distributed in 24 linkage groups for a total length of 1,272 cM. Through bioinformatic approaches we have analysed 327 DArT clones to reveal their redundancy, syntenic and functional aspects. The DNA sequences of 174 DArT markers were assembled into a non-redundant set of 60 marker clusters. This explained the generation of clusters in very small chromosome regions across genomes. Of these DArT markers, 61 showed highly significant (Expectation < E-10) BLAST similarity to gene sequences in public databases of model species such as Brachypodium and rice. Based on sequence alignments, the analysis revealed a mosaic gene conservation, with 54 and 72 genes present in rice and Brachypodium species, respectively.

conclusionsIn the present manuscript we provide a detailed DArT markers characterization and the basis for future efforts in durum wheat map comparing.

Indexed as

Chromosome MappingGenetic MarkersGenome, PlantBrachypodiumComputational BiologyExpressed Sequence TagsGenetic LinkageGenotypeHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsOryzaTriticumGenetic Markers

Identifiers

PMID24304553
PMCPMC3866978

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