ArticleACS chemical neuroscience2014
Wavelength-selective one- and two-photon uncaging of GABA.
Article in ACS chemical neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Review
- Intracellular delivery and deep tissue penetration of nucleoside triphosphates using photocleavable covalently bound dendritic polycations.Chemical science · 2024Article
- The Development and Application of Opto-Chemical Tools in the Zebrafish.Molecules (Basel, Switzerland) · 2022Review
- Caged lipids for subcellular manipulation.Current opinion in chemical biology · 2021Review
- Article
- Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.Chemical reviews · 2020Article
- Thiocoumarin Caged Nucleotides: Synthetic Access and Their Photophysical Properties.Molecules (Basel, Switzerland) · 2020Article
- Useful Caged Compounds for Cell Physiology.Accounts of chemical research · 2020Article
- Precise 3D modulation of electro-optical parameters during neurotransmitter uncaging experiments with neurons in vitro.Scientific reports · 2020Article
- Optofluidic control of rodent learning using cloaked caged glutamate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Spacer-Mediated Control of Coumarin Uncaging for Photocaged Thymidine.The Journal of organic chemistry · 2020Article
- Photopotentiation of the GABAProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Optical control of neuronal ion channels and receptors.Nature reviews. Neuroscience · 2019Review
- Development of photolabile protecting groups and their application to the optochemical control of cell signaling.Current opinion in structural biology · 2019Review
- Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation.Methods in enzymology · 2019Article
- Optical probing of acetylcholine receptors on neurons in the medial habenula with a novel caged nicotine drug analogue.The Journal of physiology · 2018Article
- Two-Photon Uncaging of Glutamate.Frontiers in synaptic neuroscience · 2018Review
- Intracellular Uncaging of cGMP with Blue Light.ACS chemical neuroscience · 2017Article
- An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation.Journal of visualized experiments : JoVE · 2017Article
- Sequential Uncaging with Green Light can be Achieved by Fine-Tuning the Structure of a Dicyanocoumarin Chromophore.ChemistryOpen · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have synthesized photolabile 7-diethylamino coumarin (DEAC) derivatives of γ-aminobutyric acid (GABA). These caged neurotransmitters efficiently release GABA using linear or nonlinear excitation. We used a new DEAC-based caging chromophore that has a vinyl acrylate substituent at the 3-position that shifts the absorption maximum of DEAC to about 450 nm and thus is named "DEAC450". DEAC450-caged GABA is photolyzed with a quantum yield of 0.39 and is highly soluble and stable in physiological buffer. We found that DEAC450-caged GABA is relatively inactive toward two-photon excitation at 720 nm, so when paired with a nitroaromatic caged glutamate that is efficiently excited at such wavelengths, we could photorelease glutamate and GABA around single spine heads on neurons in brain slices with excellent wavelength selectivity using two- and one-photon photolysis, respectively. Furthermore, we found that DEAC450-caged GABA could be effectively released using two-photon excitation at 900 nm with spatial resolution of about 3 μm. Taken together, our experiments show that the DEAC450 caging chromophore holds great promise for the development of new caged compounds that will enable wavelength-selective, two-color interrogation of neuronal signaling with excellent subcellular resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.