ArticlePloS one2013
A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- Human iPSC Reprogramming Success: The Impact of Approaches and Source Materials.Stem cells international · 2025Article
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- Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.Annals of translational medicine · 2021Article
- Addressing Manufacturing Challenges for Commercialization of iPSC-Based Therapies.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Cellular reprogramming: Mathematics meets medicine.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Human Induced Pluripotent Stem Cells : Clinical Significance and Applications in Neurologic Diseases.Journal of Korean Neurosurgical Society · 2019Article
- Osteopetrotic induced pluripotent stem cells derived from patients with different disease-associated mutations by non-integrating reprogramming methods.Stem cell research & therapy · 2019Article
- Computational Analysis of Altering Cell Fate.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Mbd3 Promotes Reprogramming of Primary Human Fibroblasts.International journal of stem cells · 2018Article
- Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming.Stem cell research & therapy · 2018Article
- Article
- Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors.Stem cells international · 2017Article
- Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications.Stem cell reviews and reports · 2016Article
- Chemicals as the Sole Transformers of Cell Fate.International journal of stem cells · 2016Review
- Looking into the future: Using induced pluripotent stem cells to build two and three dimensional ocular tissue for cell therapy and disease modeling.Brain research · 2016Review
- Robust Differentiation of mRNA-Reprogrammed Human Induced Pluripotent Stem Cells Toward a Retinal Lineage.Stem cells translational medicine · 2016Article
- Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions.Cellular and molecular life sciences : CMLS · 2016Article
- Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method.Stem cells international · 2016Article
- A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production.Stem cell reviews and reports · 2015Article
- cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications.Stem cell reports · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A systematic evaluation of three different methods for generating induced pluripotent stem (iPS) cells was performed using the same set of parental cells in our quest to develop a feeder independent and xeno-free method for somatic cell reprogramming that could be transferred into a GMP environment. When using the BJ fibroblast cell line, the highest reprogramming efficiency (1.89% of starting cells) was observed with the mRNA based method which was almost 20 fold higher than that observed with the retrovirus (0.2%) and episomal plasmid (0.10%) methods. Standard characterisation tests did not reveal any differences in an array of pluripotency markers between the iPS lines derived using the various methods. However, when the same methods were used to reprogram three different primary fibroblasts lines, two derived from patients with rapid onset parkinsonism dystonia and one from an elderly healthy volunteer, we consistently observed higher reprogramming efficiencies with the episomal plasmid method, which was 4 fold higher when compared to the retroviral method and over 50 fold higher than the mRNA method. Additionally, with the plasmid reprogramming protocol, recombinant vitronectin and synthemax® could be used together with commercially available, fully defined, xeno-free essential 8 medium without significantly impacting the reprogramming efficiency. To demonstrate the robustness of this protocol, we reprogrammed a further 2 primary patient cell lines, one with retinosa pigmentosa and the other with Parkinsons disease. We believe that we have optimised a simple and reproducible method which could be used as a starting point for developing GMP protocols, a prerequisite for generating clinically relevant patient specific iPS cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.