ArticleThe Journal of biological chemistry2014
A novel acidic matrix protein, PfN44, stabilizes magnesium calcite to inhibit the crystallization of aragonite.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 74 citations in OpenAlex.
- Egg capsule mineralization via vaterite transportation in the invasive apple snail Pomacea canaliculata.Molecular biology and evolution · 2026Article
- Exploring proteins within the coccolith matrix.Scientific reports · 2024Article
- Directional fabrication and dissolution of larval and juvenile oyster shells under ocean acidification.Proceedings. Biological sciences · 2023Article
- Transcriptome analysis of the bivalve Placuna placenta mantle reveals potential biomineralization-related genes.Scientific reports · 2022Article
- Transcriptomic analysis of shell repair and biomineralization in the blue mussel, Mytilus edulis.BMC genomics · 2021Article
- PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation.Marine biotechnology (New York, N.Y.) · 2021Article
- Recombinant transgelin-like protein 1 from Mytilus shell induces formation of CaCOFEBS open bio · 2020Article
- Mineral formation in the primary polyps of pocilloporoid corals.Acta biomaterialia · 2019Article
- A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization.The Journal of biological chemistry · 2019Article
- Piwi-interacting RNA (piRNA) expression patterns in pearl oyster (Pinctada fucata) somatic tissues.Scientific reports · 2019Article
- Transcriptional regulation of the matrix protein Shematrin-2 during shell formation in pearl oyster.The Journal of biological chemistry · 2018Article
- Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.Scientific reports · 2018Article
- Hemocytes in the extrapallial space of Pinctada fucata are involved in immunity and biomineralization.Scientific reports · 2018Article
- fam20C participates in the shell formation in the pearl oyster, Pinctada fucata.Scientific reports · 2018Article
- The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.Scientific reports · 2017Article
- A Novel Matrix Protein, PfY2, Functions as a Crucial Macromolecule during Shell Formation.Scientific reports · 2017Article
- Heterogeneous distribution of dye-labelled biomineralizaiton proteins in calcite crystals.Scientific reports · 2015Article
- In-depth proteomic analysis of shell matrix proteins of Pinctada fucata.Scientific reports · 2015Article
- Extensible byssus of Pinctada fucata: Ca(2+)-stabilized nanocavities and a thrombospondin-1 protein.Scientific reports · 2015Article
- Microarray: a global analysis of biomineralization-related gene expression profiles during larval development in the pearl oyster, Pinctada fucata.BMC genomics · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnesium is widely used to control calcium carbonate deposition in the shell of pearl oysters. Matrix proteins in the shell are responsible for nucleation and growth of calcium carbonate crystals. However, there is no direct evidence supporting a connection between matrix proteins and magnesium. Here, we identified a novel acidic matrix protein named PfN44 that affected aragonite formation in the shell of the pearl oyster Pinctada fucata. Using immunogold labeling assays, we found PfN44 in both the nacreous and prismatic layers. In shell repair, PfN44 was repressed, whereas other matrix proteins were up-regulated. Disturbing the function of PfN44 by RNAi led to the deposition of porous nacreous tablets with overgrowth of crystals in the nacreous layer. By in vitro circular dichroism spectra and fluorescence quenching, we found that PfN44 bound to both calcium and magnesium with a stronger affinity for magnesium. During in vitro calcium carbonate crystallization and calcification of amorphous calcium carbonate, PfN44 regulated the magnesium content of crystalline carbonate polymorphs and stabilized magnesium calcite to inhibit aragonite deposition. Taken together, our results suggested that by stabilizing magnesium calcite to inhibit aragonite deposition, PfN44 participated in P. fucata shell formation. These observations extend our understanding of the connections between matrix proteins and magnesium.
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