ArticlePlant physiology2014
Double knockout mutants of Arabidopsis grown under normal conditions reveal that the plastidial phosphorylase isozyme participates in transitory starch metabolism.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 83 citations in OpenAlex.
- Protein targeting to Starch 2 and the plastidial phosphorylase 1 revealed protein-protein interactions with photosynthesis proteins in yeast two-hybrid screenings.Plant signaling & behavior · 2025Article
- Transcriptomics analysis for elucidating the mechanism underlying the enhancement of Arabidopsis Thaliana growth by the endophytic fungus Clonostachys rosea.Archives of microbiology · 2025Article
- Old player, new roles: defining the role of the plastidial phosphorylase.The New phytologist · 2025Review
- Plastidial starch phosphorylase regulates maltodextrin turnover during starch granule initiation in Arabidopsis leaves.Plant physiology · 2025Article
- The plastidial α-glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- Branched oligosaccharides cause atypical starch granule initiation in Arabidopsis chloroplasts.Plant physiology · 2025Article
- Wheat MYOSIN-RESEMBLING CHLOROPLAST PROTEIN controls B-type starch granule initiation timing during endosperm development.Plant physiology · 2024Article
- Arabidopsis MORC1 and MED9 Interact to Regulate Defense Gene Expression and Plant Fitness.The plant pathology journal · 2024Article
- Starch phosphorylation-A needle in a haystack.Plant methods · 2024Article
- Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1.The Plant cell · 2023Article
- Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis.BMC plant biology · 2023Article
- Split selectable marker systems utilizing inteins facilitate gene stacking in plants.Communications biology · 2023Article
- The Plastidial Glucan Phosphorylase Affects the Maltooligosaccharide Metabolism in Parenchyma Cells of Potato (Solanum tuberosum L.) Tuber Discs.Plant & cell physiology · 2023Article
- Transcriptomic analysis of mesocarp tissue during fruit development of the oil palm revealed specific isozymes related to starch metabolism that control oil yield.Frontiers in plant science · 2023Article
- Starch degradation in the bean fruit pericarp is characterized by an increase in maltose metabolism.Physiologia plantarum · 2022Article
- Loss of PROTEIN TARGETING TO STARCH 2 has variable effects on starch synthesis across organs and species.Journal of experimental botany · 2022Article
- Article
- Molecular Functions and Pathways of Plastidial Starch Phosphorylase (PHO1) in Starch Metabolism: Current and Future Perspectives.International journal of molecular sciences · 2021Review
- Starch Granule Size and Morphology ofMolecules (Basel, Switzerland) · 2021Article
- Starch Granules inInternational journal of molecular sciences · 2021Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In leaves of two starch-related single-knockout lines lacking either the cytosolic transglucosidase (also designated as disproportionating enzyme 2, DPE2) or the maltose transporter (MEX1), the activity of the plastidial phosphorylase isozyme (PHS1) is increased. In both mutants, metabolism of starch-derived maltose is impaired but inhibition is effective at different subcellular sites. Two constitutive double knockout mutants were generated (designated as dpe2-1×phs1a and mex1×phs1b) both lacking functional PHS1. They reveal that in normally grown plants, the plastidial phosphorylase isozyme participates in transitory starch degradation and that the central carbon metabolism is closely integrated into the entire cell biology. All plants were grown either under continuous illumination or in a light-dark regime. Both double mutants were compromised in growth and, compared with the single knockout plants, possess less average leaf starch when grown in a light-dark regime. Starch and chlorophyll contents decline with leaf age. As revealed by transmission electron microscopy, mesophyll cells degrade chloroplasts, but degradation is not observed in plants grown under continuous illumination. The two double mutants possess similar but not identical phenotypes. When grown in a light-dark regime, mesophyll chloroplasts of dpe2-1×phs1a contain a single starch granule but under continuous illumination more granules per chloroplast are formed. The other double mutant synthesizes more granules under either growth condition. In continuous light, growth of both double mutants is similar to that of the parental single knockout lines. Metabolite profiles and oligoglucan patterns differ largely in the two double mutants.
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