ArticleeLife2013
Mechanism of chiral proofreading during translation of the genetic code.
Article in eLife, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Chiral methionine oxidation reagents reveal stereospecific proteome modifications.bioRxiv : the preprint server for biology · 2026Article
- Chirality Makes or Breaks Chemically Driven Self-Assembly.Angewandte Chemie (International ed. in English) · 2025Article
- DTD1 modulates synaptic efficacy by maintaining D-serine and D-aspartate homeostasis.Science China. Life sciences · 2025Article
- When Paul Berg meets Donald Crothers: an achiral connection through protein biosynthesis.Nucleic acids research · 2024Article
- A translation proofreader of archaeal origin imparts multi-aldehyde stress tolerance to land plants.eLife · 2024Article
- Distinct localization of chiral proofreaders resolves organellar translation conflict in plants.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Design principles and functional basis of enantioselectivity of alanyl-tRNA synthetase and a chiral proofreader during protein biosynthesis.Nucleic acids research · 2023Article
- Co-evolution of the translation apparatus and eukaryotes.Journal of biosciences · 2023Article
- Biological effects of the loss of homochirality in a multicellular organism.Nature communications · 2022Article
- Switching a conflicted bacterial DTD-tRNA code is essential for the emergence of mitochondria.Science advances · 2022Article
- Recruitment of archaeal DTD is a key event toward the emergence of land plants.Science advances · 2021Article
- Chiral discrimination in a mutated IDH enzymatic reaction in cancer: a computational perspective.European biophysics journal : EBJ · 2020Article
- Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia.eLife · 2020Article
- Chiral checkpoints during protein biosynthesis.The Journal of biological chemistry · 2019Review
- Article
- A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia.Nature communications · 2018Article
- Fluorothreonyl-tRNA deacylase prevents mistranslation in the organofluorine producerProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.Molecules (Basel, Switzerland) · 2016Review
- Elongation Factor Tu Prevents Misediting of Gly-tRNA(Gly) Caused by the Design Behind the Chiral Proofreading Site of D-Aminoacyl-tRNA Deacylase.PLoS biology · 2016Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological macromolecular world is homochiral and effective enforcement and perpetuation of this homochirality is essential for cell survival. In this study, we present the mechanistic basis of a configuration-specific enzyme that selectively removes D-amino acids erroneously coupled to tRNAs. The crystal structure of dimeric D-aminoacyl-tRNA deacylase (DTD) from Plasmodium falciparum in complex with a substrate-mimicking analog shows how it uses an invariant 'cross-subunit' Gly-cisPro dipeptide to capture the chiral centre of incoming D-aminoacyl-tRNA. While no protein residues are directly involved in catalysis, the unique side chain-independent mode of substrate recognition provides a clear explanation for DTD's ability to act on multiple D-amino acids. The strict chiral specificity elegantly explains how the enriched cellular pool of L-aminoacyl-tRNAs escapes this proofreading step. The study thus provides insights into a fundamental enantioselection process and elucidates a chiral enforcement mechanism with a crucial role in preventing D-amino acid infiltration during the evolution of translational apparatus. DOI: http://dx.doi.org/10.7554/eLife.01519.001.
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