ArticleJournal of visualized experiments : JoVE2013
High-throughput flow cytometry cell-based assay to detect antibodies to N-methyl-D-aspartate receptor or dopamine-2 receptor in human serum.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- T cell reactivity to regulatory factor X4 in type 1 narcolepsy.Scientific reports · 2021Article
- Pro-inflammatory dopamine-2 receptor-specific T cells in paediatric movement and psychiatric disorders.Clinical & translational immunology · 2020Article
- Effects of the Positive Threshold and Data Analysis on Human MOG Antibody Detection by Live Flow Cytometry.Frontiers in immunology · 2020Article
- Characterization of the human myelin oligodendrocyte glycoprotein antibody response in demyelination.Acta neuropathologica communications · 2019Article
- Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases.PloS one · 2017Article
- Dopamine-2 receptor extracellular N-terminus regulates receptor surface availability and is the target of human pathogenic antibodies from children with movement and psychiatric disorders.Acta neuropathologica communications · 2016Article
- Autoantibodies to central nervous system neuronal surface antigens: psychiatric symptoms and psychopharmacological implications.Psychopharmacology · 2016Review
- Autoimmune encephalitis in psychiatric institutions: current perspectives.Neuropsychiatric disease and treatment · 2016Review
- Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System.Frontiers in molecular neuroscience · 2016Review
- Antibodies to myelin oligodendrocyte glycoprotein in bilateral and recurrent optic neuritis.Neurology(R) neuroimmunology & neuroinflammation · 2014Article
- Antibodies to MOG have a demyelination phenotype and affect oligodendrocyte cytoskeleton.Neurology(R) neuroimmunology & neuroinflammation · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the recent years, antibodies against surface and conformational proteins involved in neurotransmission have been detected in autoimmune CNS diseases in children and adults. These antibodies have been used to guide diagnosis and treatment. Cell-based assays have improved the detection of antibodies in patient serum. They are based on the surface expression of brain antigens on eukaryotic cells, which are then incubated with diluted patient sera followed by fluorochrome-conjugated secondary antibodies. After washing, secondary antibody binding is then analyzed by flow cytometry. Our group has developed a high-throughput flow cytometry live cell-based assay to reliably detect antibodies against specific neurotransmitter receptors. This flow cytometry method is straight forward, quantitative, efficient, and the use of a high-throughput sampler system allows for large patient cohorts to be easily assayed in a short space of time. Additionally, this cell-based assay can be easily adapted to detect antibodies to many different antigenic targets, both from the central nervous system and periphery. Discovering additional novel antibody biomarkers will enable prompt and accurate diagnosis and improve treatment of immune-mediated disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.