ArticleJournal of visualized experiments : JoVE2013
Organelle transport in cultured Drosophila cells: S2 cell line and primary neurons.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- PolyQ-Expansion of Ataxin-2 Disrupts Microtubule Stability and Impairs Axon Outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- 'Mitotic' kinesin-5 is a dynamic brake for axonal growth in Drosophila.Development (Cambridge, England) · 2025Article
- Detection of Human GPCR Activity in Drosophila S2 Cells Using the Tango System.International journal of molecular sciences · 2024Article
- An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.Journal of cell science · 2024Article
- Cellular mechanisms underlying carry-over effects after magnetic stimulation.Scientific reports · 2024Article
- Tubulin CFEOM mutations both inhibit or activate kinesin motor activity.Molecular biology of the cell · 2024Article
- CryoET shows cofilactin filaments inside the microtubule lumen.EMBO reports · 2023Article
- CryoET shows cofilactin filaments inside the microtubule lumen.bioRxiv : the preprint server for biology · 2023Article
- Article
- Kinetochore protein Spindly controls microtubule polarity inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- TheBiology open · 2019Article
- Ooplasmic flow cooperates with transport and anchorage inThe Journal of cell biology · 2018Article
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Microtubule-microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Engineered kinesin motor proteins amenable to small-molecule inhibition.Nature communications · 2016Article
- Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.Journal of cell science · 2016Article
- Article
- Kinesin-1-powered microtubule sliding initiates axonal regeneration in Drosophila cultured neurons.Molecular biology of the cell · 2015Article
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Authors and funding
3 authors.
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Abstract
Drosophila S2 cells plated on a coverslip in the presence of any actin-depolymerizing drug form long unbranched processes filled with uniformly polarized microtubules. Organelles move along these processes by microtubule motors. Easy maintenance, high sensitivity to RNAi-mediated protein knock-down and efficient procedure for creating stable cell lines make Drosophila S2 cells an ideal model system to study cargo transport by live imaging. The results obtained with S2 cells can be further applied to a more physiologically relevant system: axonal transport in primary neurons cultured from dissociated Drosophila embryos. Cultured neurons grow long neurites filled with bundled microtubules, very similar to S2 processes. Like in S2 cells, organelles in cultured neurons can be visualized by either organelle-specific fluorescent dyes or by using fluorescent organelle markers encoded by DNA injected into early embryos or expressed in transgenic flies. Therefore, organelle transport can be easily recorded in neurons cultured on glass coverslips using living imaging. Here we describe procedures for culturing and visualizing cargo transport in Drosophila S2 cells and primary neurons. We believe that these protocols make both systems accessible for labs studying cargo transport.
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