ArticleJournal of visualized experiments : JoVE2013
Use of Galleria mellonella as a model organism to study Legionella pneumophila infection.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Galleria mellonella as a novel invertebrate model for studying Ehrlichia ruminantium pathogenesis and host-pathogen interactions.FEMS microbiology letters · 2026Article
- Bactericidal activity of mammalian histones is caused by large membrane pore formation.Cell reports · 2025Article
- Oxidative, Genotoxic and Epigenotoxic Effects of Pimpla turionellae Venom at Pharmacological Perspective.Neotropical entomology · 2025Article
- Virulence profile of carbapenem-resistantMicrobiology spectrum · 2025Article
- Dissecting the inhibitory activity of Burkholderia orbicola against Gram-positive and - negative multidrug-resistant bacteria.PloS one · 2025Article
- In Vitro and In Vivo Studies on a Mononuclear Ruthenium Complex Reveals It is a Highly Effective, Fast-Acting, Broad-Spectrum Antimicrobial in Physiologically Relevant Conditions.ACS infectious diseases · 2024Article
- Evaluation ofMicrobial genomics · 2024Article
- Stenotrophomonas maltophilia uses a c-di-GMP module to sense the mammalian body temperature during infection.PLoS pathogens · 2024Article
- Bioactivity of silverleaf nightshade (Solanum elaeagnifolium Cav.) berries parts against Galleria mellonella and Erwinia carotovora and LC-MS chemical profile of its potential extract.Scientific reports · 2024Article
- An Effective Modification Strategy to Build Multifunctional Peptides Based on a Trypsin Inhibitory Peptide of the Kunitz Family.Pharmaceutics · 2024Article
- Animal models ofFrontiers in microbiology · 2024Review
- Predicting bacterial interaction outcomes from monoculture growth and supernatant assays.ISME communications · 2024Article
- Ruthenium(II)-Arene Curcuminoid Complexes as Photosensitizer Agents for Antineoplastic and Antimicrobial Photodynamic Therapy: In Vitro and In Vivo Insights.Molecules (Basel, Switzerland) · 2023Article
- Negative interactions and virulence differences drive the dynamics in multispecies bacterial infections.Proceedings. Biological sciences · 2023Article
- The VirtuousAntibiotics (Basel, Switzerland) · 2023Review
- Preliminary Toxicity Evaluation of a Porphyrin Photosensitizer in an Alternative Preclinical Model.International journal of molecular sciences · 2023Article
- Use of the waxworm Galleria mellonella larvae as an infection model to study Acinetobacter baumannii.PloS one · 2023Article
- Larval gut microbiome of Pelidnota luridipes (Coleoptera: Scarabaeidae): high bacterial diversity, different metabolic profiles on gut chambers and species with probiotic potential.World journal of microbiology & biotechnology · 2022Article
- Proteome Exploration ofMicrobiology spectrum · 2022Article
- Isoquinoline Antimicrobial Agent: Activity against Intracellular Bacteria and Effect on Global Bacterial Proteome.Molecules (Basel, Switzerland) · 2022Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
Legionella pneumophila, the causative agent of a severe pneumonia named Legionnaires' disease, is an important human pathogen that infects and replicates within alveolar macrophages. Its virulence depends on the Dot/Icm type IV secretion system (T4SS), which is essential to establish a replication permissive vacuole known as the Legionella containing vacuole (LCV). L. pneumophila infection can be modeled in mice however most mouse strains are not permissive, leading to the search for novel infection models. We have recently shown that the larvae of the wax moth Galleria mellonella are suitable for investigation of L. pneumophila infection. G. mellonella is increasingly used as an infection model for human pathogens and a good correlation exists between virulence of several bacterial species in the insect and in mammalian models. A key component of the larvae's immune defenses are hemocytes, professional phagocytes, which take up and destroy invaders. L. pneumophila is able to infect, form a LCV and replicate within these cells. Here we demonstrate protocols for analyzing L. pneumophila virulence in the G. mellonella model, including how to grow infectious L. pneumophila, pretreat the larvae with inhibitors, infect the larvae and how to extract infected cells for quantification and immunofluorescence microscopy. We also describe how to quantify bacterial replication and fitness in competition assays. These approaches allow for the rapid screening of mutants to determine factors important in L. pneumophila virulence, describing a new tool to aid our understanding of this complex pathogen.
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