ArticleGenome research2014
Independent RNA polymerase II preinitiation complex dynamics and nucleosome turnover at promoter sites in vivo.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Live-cell single-molecule dynamics of eukaryotic RNA polymerase machineries.Science (New York, N.Y.) · 2026Article
- Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.Molecular cell · 2021Article
- Genome-wide off-rates reveal how DNA binding dynamics shape transcription factor function.Molecular systems biology · 2020Article
- Histone exchange is associated with activator function at transcribed promoters and with repression at histone loci.Science advances · 2020Article
- Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction.eLife · 2020Article
- Article
- Challenges and guidelines toward 4D nucleome data and model standards.Nature genetics · 2018Review
- An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking.Bio-protocol · 2018Article
- Understanding nucleosome dynamics and their links to gene expression and DNA replication.Nature reviews. Molecular cell biology · 2017Review
- RNA synthesis is associated with multiple TBP-chromatin binding events.Scientific reports · 2017Article
- Chromatin Association of Gcn4 Is Limited by Post-translational Modifications Triggered by its DNA-Binding in Saccharomyces cerevisiae.Genetics · 2016Article
- Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex.eLife · 2016Article
- Global analysis of physical and functional RNA targets of hnRNP L reveals distinct sequence and epigenetic features of repressed and enhanced exons.RNA (New York, N.Y.) · 2015Article
- Uncoupling histone turnover from transcription-associated histone H3 modifications.Nucleic acids research · 2015Article
- Biased allelic expression in human primary fibroblast single cells.American journal of human genetics · 2015Article
- Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method.Methods (San Diego, Calif.) · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription by all three eukaryotic RNA polymerases involves the assembly of a large preinitiation complex (PIC) at gene promoters. The PIC comprises several general transcription factors (GTFs), including TBP, and the respective RNA polymerase. It has been suggested that some GTFs remain stably bound at active promoters to facilitate multiple transcription events. Here we used two complementary approaches to show that, in G1-arrested yeast cells, TBP exchanges very rapidly even at the most highly active RNA Pol II promoters. A similar situation is observed at RNA Pol III promoters. In contrast, TBP remains stably bound at RNA Pol I promoters. We also provide evidence that, unexpectedly, PIC dynamics are neither the cause nor the consequence of nucleosome exchange at most of the RNA Pol II promoters we analyzed. These results point to a stable reinitiation complex at RNA Pol I promoters and suggest independent PIC and nucleosome turnover at many RNA Pol II promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.