Evidence map›Paper›PMID 24298027›Full record

ArticleCold Spring Harbor protocols2013

Combining Ca2+ and membrane potential imaging in single neurons.

Marco Canepari, Kaspar E Vogt, Michel De Waard, Dejan Zecevic

Open access · greenAbstract read
In one paragraph

Article in Cold Spring Harbor protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Marco CanepariInserm, U836, Team 3, BP 170, Grenoble cedex 09, F-38042, France;
Kaspar E Vogt
Michel De Waard
Dejan Zecevic
Université Joseph Fourier · FRUniversity of Basel · CHYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability to monitor Ca(2+) signals and membrane potential simultaneously at multiple locations on the same neuron facilitates further progress in our understanding of neuronal function. In particular, this method allows correlation of electrical and chemical signals from multiple sites, including those inaccessible to microelectrodes. This protocol describes a procedure for loading cells with two indicators, a Ca(2+)-sensitive Fura dye and voltage-sensitive JPW1114, together with the equipment required for detecting and imaging the two signals. Potential problems are discussed as well as the capabilities and limitations of the technique.

Indexed as

Membrane PotentialsAnimalsCalciumCations, DivalentElectrophysiological PhenomenaFluorescent DyesNeuronsOptical ImagingStaining and LabelingCalciumCations, DivalentFluorescent Dyes

Identifiers

PMID24298027
PMCPMC5225264
OpenAlexW2070742703

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.