ArticleExtremophiles : life under extreme conditions2014
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake.
Article in Extremophiles : life under extreme conditions, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 14 citations in OpenAlex.
- The root system of Pappostipa frigida as a hotspot of active bacterial communities in the Andean steppe of the Atacama desert.Environmental microbiome · 2026Article
- Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family NaFrontiers in microbiology · 2020Article
- Critical Functions of Region 1-67 and Helix XIII in Retaining the Active Structure of NhaD Antiporter inFrontiers in microbiology · 2018Article
- Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein FromFrontiers in microbiology · 2018Article
- An Uncharacterized Major Facilitator Superfamily Transporter FromFrontiers in microbiology · 2018Article
- Characterization of a novel two-component NaScientific reports · 2017Article
- A UPF0118 family protein with uncharacterized function from the moderate halophile Halobacillus andaensis represents a novel class of NaScientific reports · 2017Article
- Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp. Y2.Extremophiles : life under extreme conditions · 2016Article
- Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis.Extremophiles : life under extreme conditions · 2014Article
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10 authors at 5 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
In this study, metagenomic DNA was screened for the Na(+)/H(+) antiporter gene from the halophilic bacteria in Daban Salt Lake by selection in Escherichia coli KNabc lacking three major Na(+)/H(+) antiporters. One gene designated as Hb_nhaD encoding a novel NhaD-type Na(+)/H(+) antiporter was finally cloned. The presence of Hb_NhaD conferred tolerance of E. coli KNabc to up to 0.5 M NaCl and 0.2 M LiCl, and an alkaline pH. Hb_NhaD has the highest identity (70.6%) with a putative NhaD-type Na(+)/H(+) antiporter from an uncharacterized Clostridiaceae species, and also has lower identity with known NhaD-type Na(+)/H(+) antiporters from Halomonas elongata (20.8%), Alkalimonas amylolytica (19.0%), Vibrio parahaemolyticus (18.9%) and Vibrio cholerae (18.7 %). pH-dependent Na(+)(Li(+))/H(+) antiport activity was detected from everted membrane vesicles prepared from E. coli KNabc carrying Hb_nhaD. Hb_NhaD exhibited very high Na(+)(Li(+))/H(+) antiport activity over a wide pH range from 6.5 to 9.0 with the highest activity at pH 7.0 which is significantly different from those of the above known NhaD-type Na(+)/H(+) antiporters. Also, the apparent K m values of Hb_NhaD for Na(+) and Li(+) at pH 7.0 were determined to be 1.31 and 2.16, respectively. Based on the above results, we proposed that Hb_NhaD should be categorized as a novel NhaD-type Na(+)/H(+) antiporter.
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