ArticleHistochemistry and cell biology2014
Probing the stiffness of isolated nucleoli by atomic force microscopy.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Regulation of transcription by R loop-nucleated transcription condensate assembly.Cell & bioscience · 2026Review
- Metabolically intact nuclei are fluidized by the activity of the chromatin remodeling motor BRG1.Biophysical journal · 2025Article
- Physiology and pharmacological targeting of phase separation.Journal of biomedical science · 2024Review
- Phase separation in immune signalling.Nature reviews. Immunology · 2022Review
- 3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers.Scientific reports · 2022Article
- Principles and functions of condensate modifying drugs.Frontiers in molecular biosciences · 2022Review
- Mechanisms and regulation underlying membraneless organelle plasticity control.Journal of molecular cell biology · 2021Review
- RNA phase separation-mediated direction of molecular trafficking under conditions of molecular crowding.Biophysical reviews · 2020Review
- Various Nucleolar Stress Inducers Result in Highly Distinct Changes in Water, Dry Mass and Elemental Content in Cancerous Cell Compartments: Investigation Using a Nano-Analytical Approach.Nanotheranostics · 2019Article
- Phase-to-Phase With Nucleoli - Stress Responses, Protein Aggregation and Novel Roles of RNA.Frontiers in cellular neuroscience · 2019Review
- Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles.Journal of molecular biology · 2018Review
- Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function.Molecular and cellular biology · 2017Article
- Are aberrant phase transitions a driver of cellular aging?BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Phase separation in biology; functional organization of a higher order.Cell communication and signaling : CCS · 2016Review
- The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology.Molecular and cellular biology · 2015Article
- The Histochemistry and Cell Biology pandect: the year 2014 in review.Histochemistry and cell biology · 2015Review
- New insights into nucleolar structure and function.F1000prime reports · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotic cells, ribosome biogenesis occurs in the nucleolus, a membraneless nuclear compartment. Noticeably, the nucleolus is also involved in several nuclear functions, such as cell cycle regulation, non-ribosomal ribonucleoprotein complex assembly, aggresome formation and some virus assembly. The most intriguing question about the nucleolus is how such dynamics processes can occur in such a compact compartment. We hypothesized that its structure may be rather flexible. To investigate this, we used atomic force microscopy (AFM) on isolated nucleoli. Surface topography imaging revealed the beaded structure of the nucleolar surface. With the AFM's ability to measure forces, we were able to determine the stiffness of isolated nucleoli. We could establish that the nucleolar stiffness varies upon drastic morphological changes induced by transcription and proteasome inhibition. Furthermore, upon ribosomal proteins and LaminB1 knockdowns, the nucleolar stiffness was increased. This led us to propose a model where the nucleolus has steady-state stiffness dependent on ribosome biogenesis activity and requires LaminB1 for its flexibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.