ArticleJournal of bacteriology2014
The MiaA tRNA modification enzyme is necessary for robust RpoS expression in Escherichia coli.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 48 citations in OpenAlex.
- MiaA-mediated tRNA modifications couple tryptophan attenuation and changes in tRNA abundance to complex phenotypes in Pseudomonas aeruginosa.Nucleic acids research · 2026Article
- tRNA-modifying enzymes in bacterial stress adaptation.Open biology · 2025Review
- The tRNA epitranscriptomic landscape and RNA modification enzymes in Vibrio cholerae.PLoS genetics · 2025Article
- Post-Transcriptional Regulation of the MiaA Prenyl Transferase by CsrA and the Small RNA CsrB inInternational journal of molecular sciences · 2025Article
- Antibiotic target discovery by integrated phenotypic and activity-based profiling of electrophilic fragments.Cell chemical biology · 2025Article
- Aminoglycoside tolerance ineLife · 2025Article
- Response ofMicroorganisms · 2024Review
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- New layers of regulation of the general stress response sigma factor RpoS.Frontiers in microbiology · 2024Review
- Arginine to glutamine mutation in the substrate binding region impaired the isopentenyl activity ofMolecular biology research communications · 2024Article
- Microbial gene expression in Guaymas Basin subsurface sediments responds to hydrothermal stress and energy limitation.The ISME journal · 2023Article
- Genome-wide screening reveals metabolic regulation of stop-codon readthrough by cyclic AMP.Nucleic acids research · 2023Article
- tRNA modification enzyme MiaB connects environmental cues to activation of Pseudomonas aeruginosa type III secretion system.PLoS pathogens · 2022Article
- A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence.Nucleic acids research · 2022Article
- MiaA (Rv2727c) mediated tRNA isopentenylation ofMolecular biology research communications · 2022Article
- Review
- Roles of Ferredoxin-Dependent Proteins in the Apicoplast of Plasmodium falciparum Parasites.mBio · 2021Article
- Bacterial tRNA 2'-O-methylation is dynamically regulated under stress conditions and modulates innate immune response.Nucleic acids research · 2020Article
- Genetic analysis of Streptomyces albus J1074 mia mutants suggests complex relationships between post-transcriptional tRNAFolia microbiologica · 2020Article
- Characterization of Autoinducer-3 Structure and Biosynthesis inACS central science · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The stationary phase/general stress response sigma factor RpoS (σ(S)) is necessary for adaptation and restoration of homeostasis in stationary phase. As a physiological consequence, its levels are tightly regulated at least at two levels. Multiple small regulatory RNA molecules modulate its translation, in a manner that is dependent on the RNA chaperone Hfq and the rpoS 5' untranslated region. ClpXP and the RssB adaptor protein degrade RpoS, unless it is protected by an anti-adaptor. We here find that, in addition to these posttranscriptional levels of regulation, tRNA modification also affects the steady-state levels of RpoS. We screened mutants of several RNA modification enzymes for an effect on RpoS expression and identified the miaA gene, encoding a tRNA isopentenyltransferase, as necessary for full expression of both an rpoS750-lacZ translational fusion and the RpoS protein. This effect is independent of rpoS, the regulatory RNAs, and RpoS degradation. RpoD steady-state levels were not significantly different in the absence of MiaA, suggesting that this is an RpoS-specific effect. The rpoS coding sequence is significantly enriched for leu codons that use MiaA-modified tRNAs, compared to rpoD and many other genes. Dependence on MiaA may therefore provide yet another way for RpoS levels to respond to growth conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.