ArticleJournal of bacteriology2014
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Mfd interacts with RNA polymerase to modulate flagellum-dependent motility, chemotaxis, and population heterogeneity in the stationary phase ofJournal of bacteriology · 2026Article
- Protein Homeostasis Impairment Alters Phenotypic Heterogeneity of Biofilm Communities.Molecular microbiology · 2025Article
- Article
- The DnaJK chaperone ofmBio · 2025Article
- SwrA-mediated Multimerization of DegU and an Upstream Activation Sequence Enhance Flagellar Gene Expression in Bacillus subtilis.Journal of molecular biology · 2024Article
- Harzianic acid exerts antimicrobial activity against Gram-positive bacteria and targets the cell membrane.Frontiers in microbiology · 2024Article
- Density and temperature controlled fluid extraction in a bacterial biofilm is determined by poly-γ-glutamic acid production.NPJ biofilms and microbiomes · 2022Article
- TnMicrobiology spectrum · 2022Article
- Identification of Genes Required for Swarming Motility inJournal of bacteriology · 2022Article
- Poly-Gamma-Glutamic Acid Secretion Protects Bacillus subtilis from Zinc and Copper Intoxication.Microbiology spectrum · 2022Article
- High-Throughput Time-Lapse Fluorescence Microscopy Screening for Heterogeneously Expressed Genes in Bacillus subtilis.Microbiology spectrum · 2022Article
- Dynamic Hybrid Flagellar Motors-Fuel Switch and More.Frontiers in microbiology · 2022Review
- Microbial hitchhiking: how Streptomyces spores are transported by motile soil bacteria.The ISME journal · 2021Article
- Mechanotransduction in Prokaryotes: A Possible Mechanism of Spaceflight Adaptation.Life (Basel, Switzerland) · 2021Article
- TnApplied and environmental microbiology · 2020Article
- A Skeptic's Guide to Bacterial Mechanosensing.Journal of molecular biology · 2020Review
- Glucose-Mediated Protein Arginine Phosphorylation/Dephosphorylation RegulatesFrontiers in microbiology · 2020Article
- Functional Regulators of Bacterial Flagella.Annual review of microbiology · 2019Review
- SwrD (YlzI) Promotes Swarming in Bacillus subtilis by Increasing Power to Flagellar Motors.Journal of bacteriology · 2018Article
- Protein lysine acetylation plays a regulatory role in Bacillus subtilis multicellularity.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bacillus subtilis swims in liquid media and swarms over solid surfaces, and it encodes two sets of flagellar stator homologs. Here, we show that B. subtilis requires only the MotA/MotB stator during swarming motility and that the residues required for stator force generation are highly conserved from the Proteobacteria to the Firmicutes. We further find that mutants that abolish stator function also result in an overproduction of the extracellular polymer poly-γ-glutamate (PGA) to confer a mucoid colony phenotype. PGA overproduction appeared to be the result of an increase in the expression of the pgs operon that encodes genes for PGA synthesis. Transposon mutagenesis was conducted to identify insertions that abolished colony mucoidy and disruptions in known transcriptional regulators of PGA synthesis (Com and Deg two-component systems) as well as mutants defective in transcription-coupled DNA repair (Mfd)-reduced expression of the pgs operon. A final class of insertions disrupted proteins involved in the assembly of the flagellar filament (FliD, FliT, and FlgL), and these mutants did not reduce expression of the pgs operon, suggesting a second mechanism of PGA control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.