ArticleJournal of biomechanics2014
Mechanical stiffness as an improved single-cell indicator of osteoblastic human mesenchymal stem cell differentiation.
Article in Journal of biomechanics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- High-throughput Genome Wide CRISPR Knock Out mechanical sort identifies genes driving metastatic cancer cell softening.bioRxiv : the preprint server for biology · 2026Article
- Unlocking the Potential of Stem Cell Microenvironments In Vitro.Bioengineering (Basel, Switzerland) · 2024Review
- A Machine Learning-Based Image Segmentation Method to Quantify In Vitro Osteoclast Culture Endpoints.Calcified tissue international · 2023Article
- Strain-dependent elastography of cancer cells reveals heterogeneity and stiffening due to attachment.Journal of biomechanics · 2023Article
- Mechanobiology: A landscape for reinterpreting stem cell heterogeneity and regenerative potential in diseased tissues.iScience · 2023Review
- Nanomaterials-Functionalized Hydrogels for the Treatment of Cutaneous Wounds.International journal of molecular sciences · 2022Review
- Instantaneous 4D micro-particle image velocimetry (µPIV) via multifocal microscopy (MUM).Scientific reports · 2022Article
- Enhanced osteogenic differentiation of alendronate-conjugated nanodiamonds for potential osteoporosis treatment.Biomaterials research · 2021Article
- The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.Science advances · 2021Article
- The Mechanical Interplay Between Differentiating Mesenchymal Stem Cells and Gelatin-Based Substrates Measured by Atomic Force Microscopy.Frontiers in cell and developmental biology · 2021Article
- Computational modelling unveils how epiblast remodelling and positioning rely on trophectoderm morphogenesis during mouse implantation.PloS one · 2021Article
- Reengineering Bone-Implant Interfaces for Improved Mechanotransduction and Clinical Outcomes.Stem cell reviews and reports · 2020Review
- Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing.Biomicrofluidics · 2018Article
- Quantification of Thrips Damage Using Ilastik and ImageJ Fiji.Bio-protocol · 2018Article
- Biophysical subsets of embryonic stem cells display distinct phenotypic and morphological signatures.PloS one · 2018Article
- Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma.International journal of oncology · 2017Article
- Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness.Scientific reports · 2017Article
- High-throughput physical phenotyping of cell differentiation.Microsystems & nanoengineering · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Although it has been established that cellular stiffness can change as a stem cell differentiates, the precise relationship between cell mechanics and other phenotypic properties remains unclear. Inherent cell heterogeneity and asynchronous differentiation complicate population analysis; therefore, single-cell analysis was employed to determine how changes in cell stiffness correlate with changes in molecular biomarkers during differentiation. Design of a custom gridded tissue culture dish facilitated single-cell comparisons between cell mechanics and other differentiation biomarkers by enabling sequential measurement of cell mechanics and protein biomarker expression at the single cell level. The Young's modulus of mesenchymal stem cells was shown not only to decrease during chemically-induced osteoblast differentiation, but also to correlate more closely with the day of differentiation than did the relative expression of the traditional osteoblast differentiation markers, bone sialoprotein and osteocalcin. Therefore, cell stiffness, a measurable property of individual cells, may serve as an improved indicator of single-cell osteoblast differentiation compared to traditional biological markers. Revelation of additional osteoblast differentiation indicators, such as cell stiffness, can improve identification and collection of starting cell populations, with applications to mesenchymal stem cell therapies and stem cell-based tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.