ArticleBiotechnology for biofuels2013
Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Antioxidant Compounds in Microalgae and Cyanobacteria: A Sustainable Source of Bioactive Molecules.Marine drugs · 2026Review
- Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production.Marine drugs · 2022Review
- Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme.Communications biology · 2021Article
- The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Emerging Technologies in Algal Biotechnology: Toward the Establishment of a Sustainable, Algae-Based Bioeconomy.Frontiers in plant science · 2020Review
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Branched-Chain Amino Acid Catabolism Impacts Triacylglycerol Homeostasis inPlant physiology · 2019Article
- High-efficiency nuclear transformation of the microalgaeBiotechnology for biofuels · 2019Article
- Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas.The Plant cell · 2018Article
- Identification of Insertion Site by RESDA-PCR inBio-protocol · 2018Article
- Heterologous expression of a thermophilic diacylglycerol acyltransferase triggers triglyceride accumulation in Escherichia coli.PloS one · 2017Article
- Saturating Light Induces Sustained Accumulation of Oil in Plastidal Lipid Droplets in Chlamydomonas reinhardtii.Plant physiology · 2016Article
- Metabolic regulation of triacylglycerol accumulation in the green algae: identification of potential targets for engineering to improve oil yield.Plant biotechnology journal · 2016Review
- Whole Genome Re-Sequencing Identifies a Quantitative Trait Locus Repressing Carbon Reserve Accumulation during Optimal Growth in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- High-throughput metabolic screening of microalgae genetic variation in response to nutrient limitation.Metabolomics : Official journal of the Metabolomic Society · 2016Article
- Microalgal lipid droplets: composition, diversity, biogenesis and functions.Plant cell reports · 2015Review
- A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants.The Plant journal : for cell and molecular biology · 2015Article
- Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.Biotechnology for biofuels · 2014Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOils produced by microalgae are precursors to biodiesel. To achieve a profitable production of biodiesel from microalgae, identification of factors governing oil synthesis and turnover is desirable. The green microalga Chlamydomonas reinhardtii is amenable to genetic analyses and has recently emerged as a model to study oil metabolism. However, a detailed method to isolate various types of oil mutants that is adapted to Chlamydomonas has not been reported.
resultsWe describe here a forward genetic approach to isolate mutants altered in oil synthesis and turnover from C. reinhardtii. It consists of a three-step screening procedure: a primary screen by flow cytometry of Nile red stained transformants grown in 96-deep-well plates under three sequential conditions (presence of nitrogen, then absence of nitrogen, followed by oil remobilization); a confirmation step using Nile red stained biological triplicates; and a validation step consisting of the quantification by thin layer chromatography of oil content of selected strains. Thirty-one mutants were isolated by screening 1,800 transformants generated by random insertional mutagenesis (1.7%). Five showed increased oil accumulation under the nitrogen-replete condition and 13 had altered oil content under nitrogen-depletion. All mutants were affected in oil remobilization.
conclusionThis study demonstrates that various types of oil mutants can be isolated in Chlamydomonas based on the method set-up here, including mutants accumulating oil under optimal biomass growth. The strategy conceived and the protocol set-up should be applicable to other microalgal species such as Nannochloropsis and Chlorella, thus serving as a useful tool in Chlamydomonas oil research and algal biotechnology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.