ArticleBMC genomics2013
Complex gene expression in the dragline silk producing glands of the Western black widow (Latrodectus hesperus).
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Strategies for Making High-Performance Artificial Spider Silk Fibers.Advanced functional materials · 2024Review
- The evolutionary history of cribellate orb-weaver capture thread spidroins.BMC ecology and evolution · 2022Article
- Egg Case Protein 3: A Constituent of Black Widow Spider Tubuliform Silk.Molecules (Basel, Switzerland) · 2021Article
- Shifts in morphology, gene expression, and selection underlie web loss in Hawaiian Tetragnatha spiders.BMC ecology and evolution · 2021Article
- Structural Characterization of Black Widow Spider Dragline Silk Proteins CRP1 and CRP4.Molecules (Basel, Switzerland) · 2020Article
- Towards engineering and production of artificial spider silk using tools of synthetic biology.Engineering biology · 2020Review
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- Evolutionary shifts in gene expression decoupled from gene duplication across functionally distinct spider silk glands.Scientific reports · 2017Article
- The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression.Nature genetics · 2017Article
- Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders.BMC evolutionary biology · 2017Article
- Comprehensive Proteomic Analysis of Spider Dragline Silk from Black Widows: A Recipe to Build Synthetic Silk Fibers.International journal of molecular sciences · 2016Article
- Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers.Journal of proteome research · 2015Article
- Spider Transcriptomes Identify Ancient Large-Scale Gene Duplication Event Potentially Important in Silk Gland Evolution.Genome biology and evolution · 2015Article
- Toward spinning artificial spider silk.Nature chemical biology · 2015Review
- Spider genomes provide insight into composition and evolution of venom and silk.Nature communications · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundOrb-web and cob-web weaving spiders spin dragline silk fibers that are among the strongest materials known. Draglines are primarily composed of MaSp1 and MaSp2, two spidroins (spider fibrous proteins) expressed in the major ampullate (MA) silk glands. Prior genetic studies of dragline silk have focused mostly on determining the sequence of these spidroins, leaving other genetic aspects of silk synthesis largely uncharacterized.
resultsHere, we used deep sequencing to profile gene expression patterns in the Western black widow, Latrodectus hesperus. We sequenced millions of 3'-anchored "tags" of cDNAs derived either from MA glands or control tissue (cephalothorax) mRNAs, then associated the tags with genes by compiling a reference database from our newly constructed normalized L. hesperus cDNA library and published L. hesperus sequences. We were able to determine transcript abundance and alternative polyadenylation of each of three loci encoding MaSp1. The ratio of MaSp1:MaSp2 transcripts varied between individuals, but on average was similar to the estimated ratio of MaSp1:MaSp2 in dragline fibers. We also identified transcription of TuSp1 in MA glands, another spidroin family member that encodes the primary component of egg-sac silk, synthesized in tubuliform glands. In addition to the spidroin paralogs, we identified 30 genes that are more abundantly represented in MA glands than cephalothoraxes and represent new candidates for involvement in spider silk synthesis.
conclusionsModulating expression rates of MaSp1 variants as well as MaSp2 and TuSp1 could lead to differences in mechanical properties of dragline fibers. Many of the newly identified candidate genes likely encode secreted proteins, suggesting they could be incorporated into dragline fibers or assist in protein processing and fiber assembly. Our results demonstrate previously unrecognized transcript complexity in spider silk glands.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.