ArticleIndian journal of virology : an official organ of Indian Virological Society2012
Prokaryotic expression and purification of highly soluble partial glycoprotein erns of Indian strain of classical Swine Fever virus.
Article in Indian journal of virology : an official organ of Indian Virological Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 16 citations in OpenAlex.
- Lentiviral-mediated delivery of classical swine fever virus Erns gene into porcine kidney-15 cells for production of recombinant ELISA diagnostic antigen.Molecular biology reports · 2019Article
- A coiled-coil motif in non-structural protein 3 (NS3) of bluetongue virus forms an oligomer.Virus genes · 2015Article
- Complete genome sequence of classical Swine Fever virus subgenogroup 2.1 from assam, India.Genome announcements · 2015Article
- Detection of cystatin C biomarker for clinical measurement of renal disease by developed ELISA diagnostic kits.Journal of translational medicine · 2014Article
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5 authors at 2 institutions in 1 country.
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Abstract
Classical swine fever (CSF) or hog cholera, caused by a positive stranded RNA virus belonging to the genus Pestivirus of the Flaviviridae family, is highly contagious and fatal disease of pigs. We report the novel design of construct for production of highly soluble glycoprotein Erns fragment using prokaryotic expression system. A truncated fragment of the Erns gene (coding for aa 109-170) denoted as 'Erns-Ag' was subcloned and expressed as hexa-histidine tag fusion on both terminus of protein in Escherichia coli. The highly soluble recombinant Erns-Ag protein with purity >95 % was purified by one step Ni-NTA affinity chromatography under native condition. Anti Erns-Ag polyclonal antibodies raised in guinea pig was found to react with CSFV antigen in infected MDCK cell line during immunoperoxidase test. The described methodology of producing a highly soluble recombinant protein with native conformation would likely to assist in development of differential diagnostic test as well as its application in raising hyperimmune sera for detection of CSFV antigen either in tissue materials or infected cell lines.
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