ArticleIndian journal of virology : an official organ of Indian Virological Society2012
Evaluation of rt-PCR assay for routine laboratory diagnosis of rabies in post mortem brain samples from different species of animals.
Article in Indian journal of virology : an official organ of Indian Virological Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Early diagnosis of rabies virus infection by RPA-CRISPR techniques in a rat model.Archives of virology · 2021Article
- Sensitive detection of the ISRSC advances · 2018Article
- Article
- Pathobiological investigation of naturally infected canine rabies cases from Sri Lanka.BMC veterinary research · 2017Article
- Diagnostic difficulties in human rabies: A case report and review of the literature.Qatar medical journal · 2016Article
- Diagnostic evaluation of RT-PCR-ELISA for the detection of rabies virus.Virusdisease · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
Rabies in domestic and wild animals continues to be a major public health threat in India. Rapid and accurate diagnosis of rabies in animals is therefore of utmost importance as the individuals who were in contact with the rabid animals are at a greater risk. A significant amount of diagnostic tissue samples submitted to our laboratory are often autolysed and the WHO recommended direct fluorescent antibody test (FAT) for rabies diagnosis cannot be used in such samples. In this pilot study we have evaluated three different diagnostic primer sets for rapid sensitive and specific detection of rabies genome from the brain samples of different species of animals. We have validated a sensitive RT-PCR assay using brain tissue samples from different species of animals such as cat, cattle, dog, mouse and human, for routine diagnosis of rabies. Our results show the potential of this assay as a confirmatory test when the FAT results are unreliable and also as an alternative diagnostic test in circumstances when the diagnostic samples are unsuitable for use in FAT. Furthermore the nucleotide sequence of nucleoprotein gene amplified using this assay can also be used for the molecular epidemiological study of the rabies viruses in India.
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