ArticleSleep2013
A subset of cholinergic mushroom body neurons requires Go signaling to regulate sleep in Drosophila.
Article in Sleep, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 48 citations in OpenAlex.
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- Alcohol induces long-lasting sleep deficits in Drosophila via subsets of cholinergic neurons.Current biology : CB · 2025Article
- Exploring the Asymmetric Body's Influence on Interval Timing Behaviors of Drosophila melanogaster.Behavior genetics · 2024Article
- Variant-to-gene mapping followed by cross-species genetic screening identifies GPI-anchor biosynthesis as a regulator of sleep.Science advances · 2023Article
- LKB1 is physiologically required for sleep from Drosophila melanogaster to the Mus musculus.Genetics · 2022Article
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- Serine metabolism in the brain regulates starvation-induced sleep suppression inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The neurobiological basis of sleep: Insights from Drosophila.Neuroscience and biobehavioral reviews · 2018Review
- Learning and Its Neural Correlates in a Virtual Environment for Honeybees.Frontiers in behavioral neuroscience · 2018Article
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- Aberrant Axonal Arborization of PDF Neurons Induced by Aβ42-Mediated JNK Activation Underlies Sleep Disturbance in an Alzheimer's Model.Molecular neurobiology · 2017Article
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- The Drosophila circuitry of sleep-wake regulation.Current opinion in neurobiology · 2017Review
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
STUDY
objectivesIdentifying the neurochemistry and neural circuitry of sleep regulation is critical for understanding sleep and various sleep disorders. Fruit flies display sleep-like behavior, sharing essential features with sleep of vertebrate. In the fruit fly's central brain, the mushroom body (MB) has been highlighted as a sleep center; however, its neurochemical nature remains unclear, and whether it promotes sleep or wake is still a topic of controversy.
designWe used a video recording system to accurately monitor the locomotor activity and sleep status. Gene expression was temporally and regionally manipulated by heat induction and the Gal4/UAS system. MEASUREMENTS AND
resultsWe found that expressing pertussis toxin (PTX) in the MB by c309-Gal4 to block Go activity led to unique sleep defects as dramatic sleep increase in daytime and fragmented sleep in nighttime. We narrowed down the c309-Gal4 expressing brain regions to the MB α/β core neurons that are responsible for the Go-mediated sleep effects. Using genetic tools of neurotransmitter-specific Gal80 and RNA interference approach to suppress acetylcholine signal, we demonstrated that these MB α/β core neurons were cholinergic and sleep-promoting neurons, supporting that Go mediates an inhibitory signal. Interestingly, we found that adjacent MB α/β neurons were also cholinergic but wake-promoting neurons, in which Go signal was also required.
conclusionOur findings in fruit flies characterized a group of sleep-promoting neurons surrounded by a group of wake-promoting neurons. The two groups of neurons are both cholinergic and use Go inhibitory signal to regulate sleep.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.