ArticlePhotosynthesis research2014
A chloroplast pump model for the CO2 concentrating mechanism in the diatom Phaeodactylum tricornutum.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Role of extracellular carbonic anhydrase in the polar diatom Fragilariopsis cylindrus.The New phytologist · 2026Article
- Resilience of Phytoplankton and Microzooplankton Communities under Ocean Alkalinity Enhancement in the Oligotrophic Ocean.Environmental science & technology · 2024Article
- Hydrogen isotope fractionation is controlled by COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2023Article
- Light-driven Proton Pumps as a Potential Regulator for Carbon Fixation in Marine Diatoms.Microbes and environments · 2023Article
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- A Competitive Advantage of Middle-Sized Diatoms From Increasing Seawater COFrontiers in microbiology · 2022Article
- Review
- Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.The Plant cell · 2020Review
- Phaeodactylum tricornutum as a model organism for testing the membrane penetrability of sulphonamide carbonic anhydrase inhibitors.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- Cross-compartment metabolic coupling enables flexible photoprotective mechanisms in the diatom Phaeodactylum tricornutum.The New phytologist · 2019Article
- Dynamic changes in carbonate chemistry in the microenvironment around single marine phytoplankton cells.Nature communications · 2018Article
- Mechanisms of carbon dioxide acquisition and COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Evolutionarily distinct strategies for the acquisition of inorganic carbon from seawater in marine diatoms.Journal of experimental botany · 2017Article
- Extracellular carbonic anhydrase: Method development and its application to natural seawater.Limnology and oceanography, methods · 2017Article
- The origin of carbon isotope vital effects in coccolith calcite.Nature communications · 2017Article
- Thylakoid luminal θ-carbonic anhydrase critical for growth and photosynthesis in the marine diatom Phaeodactylum tricornutum.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Large variation in the Rubisco kinetics of diatoms reveals diversity among their carbon-concentrating mechanisms.Journal of experimental botany · 2016Article
- The ins and outs of CO2.Journal of experimental botany · 2016Article
- The minimal CO2-concentrating mechanism of Prochlorococcus spp. MED4 is effective and efficient.Plant physiology · 2014Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prior analysis of inorganic carbon (Ci) fluxes in the diatom Phaeodactylum tricornutum has indicated that transport of Ci into the chloroplast from the cytoplasm is the major Ci flux in the cell and the primary driving force for the CO2 concentrating mechanism (CCM). This flux drives the accumulation of Ci in the chloroplast stroma and generates a CO2 deficit in the cytoplasm, inducing CO2 influx into the cell. Here, the "chloroplast pump" model of the CCM in P. tricornutum is formalized and its consistency with data on CO2 and HCO3 (-) uptake rates, carbonic anhydrase (CA) activity, intracellular Ci concentration, intracellular pH, and RubisCO characteristics is assessed. The chloroplast pump model can account for the major features of the data. Analysis of photosynthetic and Ci uptake rates as a function of external Ci concentration shows that the model has the most difficulty obtaining sufficiently low cytoplasmic CO2 concentrations to support observed CO2 uptake rates at low external Ci concentrations and achieving high rates of photosynthesis. There are multiple ways in which model parameters can be varied, within a plausible range, to match measured rates of photosynthesis and CO2 uptake. To increase CO2 uptake rates, CA activity can be increased, kinetic characteristics of the putative chloroplast pump can be enhanced to increase HCO3 (-) export, or the cytoplasmic pH can be raised. To increase the photosynthetic rate, the permeability of the pyrenoid to CO2 can be reduced or RubisCO content can be increased.
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