Evidence map›Paper›PMID 24291815›Full record

ArticleNature biotechnology2014

Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.

Sarah X L Huang, Mohammad Naimul Islam, John O'Neill, Zheng Hu, Yong-Guang Yang, Ya-Wen Chen, Melanie Mumau, Michael D Green, Gordana Vunjak-Novakovic, Jahar Bhattacharya and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 325 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
325citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

325 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
  2. Frontiers in immunology · 2021
    Pooled it
  3. Pooled it
  4. Article
  5. Preclinical human models of primary ciliary dyskinesia.European respiratory review : an official journal of the European Respiratory Society · 2026
    Review
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. Article

265 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sarah X L Huang1] Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA. [2] Department of Medicine, Columbia University Medical Center, New York, New York, USA.
Mohammad Naimul IslamDepartment of Medicine, Columbia University Medical Center, New York, New York, USA.
John O'NeillDepartment of Biomedical Engineering, Columbia University, New York, New York, USA.
Zheng HuColumbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
Yong-Guang Yang1] Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA. [2] Department of Medicine, Columbia University Medical Center, New York, New York, USA.
Ya-Wen Chen1] Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA. [2] Department of Medicine, Columbia University Medical Center, New York, New York, USA.
Melanie Mumau1] Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA. [2] Department of Medicine, Columbia University Medical Center, New York, New York, USA.
Michael D GreenColumbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
Gordana Vunjak-Novakovic1] Department of Medicine, Columbia University Medical Center, New York, New York, USA. [2] Department of Biomedical Engineering, Columbia University, New York, New York, USA.
Jahar Bhattacharya1] Department of Medicine, Columbia University Medical Center, New York, New York, USA. [2] Department of Physiology & Cellular Biophysics, Columbia University Medical Center, New York, New York, USA.
Hans-Willem Snoeck1] Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA. [2] Department of Medicine, Columbia University Medical Center, New York, New York, USA. [3] Department of Microbiology and Immunology, Columbia University Medical Center, New York, New York, USA.

Funding

Bioengineering a Chimeric Human LungR01HL120046 · NHLBI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SNOECK, HANS-WILLEM E, VUNJAK-NOVAKOVIC, GORDANA · 2013 to 2022
$6.1M
Role of CD47 in xenograft rejection by macrophagesR01AI064569 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI YANG, YONG-GUANG · 2008 to 2016
$3.1M
Columbia University Graduate Training Program in Microbiology and ImmunologyT32AI106711 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Nicholas Arpaia, Uttiya Basu · 2014 to 2026
$2.1M
NHLBI NIH HHS R01 HL120046NIAID NIH HHS R01 AI064569NIAID NIH HHS T32 AI106711
6 · The paper itself

Abstract

The ability to generate lung and airway epithelial cells from human pluripotent stem cells (hPSCs) would have applications in regenerative medicine, modeling of lung disease, drug screening and studies of human lung development. We have established, based on developmental paradigms, a highly efficient method for directed differentiation of hPSCs into lung and airway epithelial cells. Long-term differentiation of hPSCs in vivo and in vitro yielded basal, goblet, Clara, ciliated, type I and type II alveolar epithelial cells. The type II alveolar epithelial cells were capable of surfactant protein-B uptake and stimulated surfactant release, providing evidence of specific function. Inhibiting or removing retinoic acid, Wnt and BMP-agonists to signaling pathways critical for early lung development in the mouse-recapitulated defects in corresponding genetic mouse knockouts. As this protocol generates most cell types of the respiratory system, it may be useful for deriving patient-specific therapeutic cells.

Indexed as

AnimalsCell DifferentiationCell LineageEpithelial CellsHumansInduced Pluripotent Stem CellsLungMiceMice, KnockoutProtein PrecursorsProteolipidsSignal TransductionTretinoinProtein PrecursorsProteolipidssurfactant protein B propeptideTretinoin

Identifiers

PMID24291815
PMCPMC4101921

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.