ArticleNature2014
Biochemical reconstitution of topological DNA binding by the cohesin ring.
Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 202 papers.
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Who cites it
202 citing papers in PubMed, 332 citations in OpenAlex.
- Nested TAD hierarchy defines cohesion zones on replicated chromosomes.The EMBO journal · 2026Article
- Cohesin promotes genomic stability by suppressing unequal sister chromatid exchange.bioRxiv : the preprint server for biology · 2026Article
- Submolecular video-imaging of the Smc5/6 complex topologically bound to DNA.Nature communications · 2026Article
- Bridging-driven condensation by eukaryotic SMC complexes is a conserved feature of genome organization.Nucleic acids research · 2026Article
- Pioneer transcription factors direct tissue-specific cohesin chromatin entry and three-dimensional genome organization.Nature genetics · 2026Article
- Interaction Between Rec8 and Mis4 Is Required for Axis-Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Distinct and compensatory roles of STAG1 and STAG2 in post-mitotic genome refolding.Nature communications · 2026Article
- Cohesin acetylation and ATPase activity control cohesion and loop architecture through distinct mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- De novo formation of cis-regulatory contacts in the absence of NIPBL-driven chromatin loop extrusion.Nature genetics · 2026Article
- Pathogenic variants in the cohesin loader subunit MAU2 underlie a distinct Cornelia de Lange Syndrome subtype.Nature communications · 2026Article
- The physical chemistry of interphase loop extrusion.Cell genomics · 2026Article
- Clinical,medRxiv : the preprint server for health sciences · 2026Article
- The role of cohesin loading at enhancers in the flux of loop extrusion and long-range transcriptional control.bioRxiv : the preprint server for biology · 2026Article
- Cohesin mutations and chromatin changes in cancer.International journal of cancer · 2026Review
- Bulk FRET Analysis of the Conformation of the Cohesin Ring.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A Biochemical Assay to Monitor the Encounter Between Cohesin and DNA Replication.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An In Vitro Assay to Study RSC Remodeling Activity Stimulation by the Cohesin Loader.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Atomic Force Microscopy Analysis of Bridging-Induced Phase Separation Mediated by SMC Proteins.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.Communications biology · 2025Article
- Cohesin Acetylation and ATPase Activity Control Cohesion and Loop Architecture through Distinct Mechanisms.bioRxiv : the preprint server for biology · 2025Article
142 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.